دورية أكاديمية
Culture of equine fibroblast-like synoviocytes on synthetic tissue scaffolds towards meniscal tissue engineering: a preliminary cell-seeding study
| العنوان: | Culture of equine fibroblast-like synoviocytes on synthetic tissue scaffolds towards meniscal tissue engineering: a preliminary cell-seeding study |
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| المؤلفون: | Warnock, Jennifer J., Fox, Derek B., Stoker, Aaron M., Beatty, Mark, Cockrell, Mary, Janicek, John C., Cook, James L. |
| بيانات النشر: | PeerJ |
| المجموعة: | ScholarsArchive@OSU (Oregon State University) |
| الوصف: | INTRODUCTION: Tissue engineering is a new methodology for addressing meniscal injury or loss. Synovium may be an ideal source of cells for in vitro meniscal fibrocartilage formation, however, favorable in vitro culture conditions for synovium must be established in order to achieve this goal. The objective of this study was to determine cellularity, cell distribution, and extracellular matrix (ECM) formation of equine fibroblast-like synoviocytes (FLS) cultured on synthetic scaffolds, for potential application in synovium-based meniscal tissue engineering. Scaffolds included open-cell poly-L-lactic acid (OPLA) sponges and polyglycolic acid (PGA) scaffolds cultured in static and dynamic culture conditions, and PGA scaffolds coated in poly-L-lactic (PLLA) in dynamic culture conditions. MATERIALS AND METHODS: Equine FLS were seeded on OPLA and PGA scaffolds, and cultured in a static environment or in a rotating bioreactor for 12 days. Equine FLS were also seeded on PGA scaffolds coated in 2% or 4% PLLA and cultured in a rotating bioreactor for 14 and 21 days. Three scaffolds from each group were fixed, sectioned and stained with Masson’s Trichrome, Safranin-O, and Hematoxylin and Eosin, and cell numbers and distribution were analyzed using computer image analysis. Three PGA and OPLA scaffolds from each culture condition were also analyzed for extracellular matrix (ECM) production via dimethylmethylene blue (sulfated glycosaminoglycan) assay and hydroxyproline (collagen) assay. PLLA coated PGA scaffolds were analyzed using double stranded DNA quantification as a reflection of cellularity and confocal laser microscopy in a fluorescent cell viability assay. RESULTS: The highest cellularity occurred in PGA constructs cultured in a rotating bioreactor, which also had a mean sulfated glycosaminoglycan content of 22.3 μg per scaffold. PGA constructs cultured in static conditions had the lowest cellularity. Cells had difficulty adhering to OPLA and the PLLA coating of PGA scaffolds; cellularity was inversely proportional ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English unknown |
| العلاقة: | https://ir.library.oregonstate.edu/concern/articles/r207tr198Test |
| الإتاحة: | https://ir.library.oregonstate.edu/concern/articles/r207tr198Test |
| حقوق: | In Copyright |
| رقم الانضمام: | edsbas.69917740 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |