دورية أكاديمية
The Effect of Media Composition on Nitrile Hydratase Activity and Stability, and on Cell Envelope Components of Rhodococcus DAP 96253
| العنوان: | The Effect of Media Composition on Nitrile Hydratase Activity and Stability, and on Cell Envelope Components of Rhodococcus DAP 96253 |
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| المؤلفون: | Tucker, Trudy-Ann Marie |
| المصدر: | Biology Dissertations |
| بيانات النشر: | ScholarWorks @ Georgia State University |
| سنة النشر: | 2008 |
| المجموعة: | Scholar Works @ Georgia State University |
| مصطلحات موضوعية: | Nitrile Hydratase, Mycolic acids, Cell envelope, Growth media, Trehalose, Rhodococcus, Biology |
| الوصف: | Rhodococcus is an important industrial organism that possesses diverse metabolic capabilities, it also has a unique cell envelope, composed of an outer layer of mycolic acids and glycolipids (free or bound lipids generally linked to the sugar trehalose). Rhodococcus is able to transform nitriles to the corresponding amide by the enzyme Nitrile Hydratase (NHase), therefore rhodococcal cells can be utilized as biocatalysts in the detoxification of nitrile waste water or in the production of industrially important amides such as acrylamide. However, the NHase within the native cells must be stable with high activity. This research examined how NHase activity and stability can be increased in native cells by changing growth media composition, the impact on the rhodococcal cell envelope was also studied. Growth media composition was altered by supplementing different sugars such as fructose, maltose or maltodextrin to replace glucose in rich solid media containing cobalt and urea for induction of NHase. The supplementation of maltose or maltodextrin resulted in significantly higher NHase activities and greater NHase stability at 55„aC. The supplementation of these different sugars was shown to alter cellular and lipid bound trehalose levels, a sugar known to stabilize proteins and a component of the rhodococcal cell envelope. Cells that had higher levels of cellular trehalose had significantly greater NHase stability at 55„aC. The effect of the different sugar supplements and inducers of NHase, such as cobalt, on cell envelope components such as mycolic acids and glycolipids were examined by High Performance Liquid Chromatography (HPLC) and Thin Layer Chromatography (TLC). The results showed that changes in mycolic acids and glycolipids occurred when the cells were grown in the presence of different sugar supplements and when the cells were induced for NHase. Susceptibility of Rhodococcus sp DAP 96253 to different antibiotics was examined to indicate if changes were occurring in the cell envelope. Differences in ... |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://scholarworks.gsu.edu/biology_diss/56Test; https://scholarworks.gsu.edu/context/biology_diss/article/1055/viewcontent/tucker_trudy_m_200712_phd.pdfTest |
| DOI: | 10.57709/1063893 |
| الإتاحة: | https://doi.org/10.57709/1063893Test https://scholarworks.gsu.edu/biology_diss/56Test https://scholarworks.gsu.edu/context/biology_diss/article/1055/viewcontent/tucker_trudy_m_200712_phd.pdfTest |
| رقم الانضمام: | edsbas.D7752DE |
| قاعدة البيانات: | BASE |
| DOI: | 10.57709/1063893 |
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