دورية أكاديمية
Phospholipase C Zeta in Human Spermatozoa: A Systematic Review on Current Development and Clinical Application
| العنوان: | Phospholipase C Zeta in Human Spermatozoa: A Systematic Review on Current Development and Clinical Application |
|---|---|
| المؤلفون: | Parrella, Alessandra, Medrano, María Llanos, Aizpurua, Jon, Gómez-Torres, María José |
| المساهمون: | Universidad de Alicante. Departamento de Biotecnología, Grupo de Inmunología, Biología Celular y del Desarrollo |
| بيانات النشر: | MDPI |
| سنة النشر: | 2024 |
| المجموعة: | RUA - Repositorio Institucional de la Universidad de Alicante |
| مصطلحات موضوعية: | PLCζ, PLCZ1, Human, Human spermatozoa, Human oocytes, Human infertility, Human oocyte activation |
| الوصف: | During fertilization, the fusion of the spermatozoa with the oocytes causes the release of calcium from the oocyte endoplasmatic reticulum. This, in turn, triggers a series of calcium ion (Ca2+) oscillations, a process known as oocyte activation. The sperm-specific factor responsible for oocyte activation is phospholipase C zeta (PLCζ). Men undergoing intracytoplasmic sperm injection (ICSI) with their spermatozoa lacking PLCζ are incapable of generating Ca2+ oscillation, leading to fertilization failure. The immunofluorescence assay is the most used technique to assess the expression and localization of PLCζ and to diagnose patients with reduced/absent ability to activate the oocytes. In these patients, the use of assisted oocyte activation (AOA) technique can help to yield successful ICSI results and shorten the time of pregnancy. However, the production of a stable PLCζ recombinant protein represents a new powerful therapeutic approach to treating individuals with this condition. We aim to conduct a systematic review focusing on the expression, level, and localization of PLCζ, discussing the novel genetic mutation associated with its impairment. In addition, we highlight the benefits of AOA, looking at new and less invasive methods to diagnose and treat cases with PLCζ dysfunction. ; This research was funded by the Cátedra Human Fertility (A-GE-Cátedra Human Fertility 35.90.6Q.00.01) and Departamento de Biotecnología of the Universidad de Alicante (VIGROB-186). |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-0067 |
| العلاقة: | https://doi.org/10.3390/ijms25021344Test; International Journal of Molecular Sciences. 2024, 25(2): 1344. https://doi.org/10.3390/ijms25021344Test; http://hdl.handle.net/10045/140410Test |
| DOI: | 10.3390/ijms25021344 |
| الإتاحة: | https://doi.org/10.3390/ijms25021344Test http://hdl.handle.net/10045/140410Test |
| حقوق: | © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0Test/). ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.6000F1BA |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14220067 |
|---|---|
| DOI: | 10.3390/ijms25021344 |