كتاب
Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum
| العنوان: | Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum |
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| المؤلفون: | Lambing, C. |
| المساهمون: | Christophe Lambing |
| بيانات النشر: | Springer Nature |
| سنة النشر: | 2022 |
| المجموعة: | Rothamsted Repository (Rothamsted Research) |
| مصطلحات موضوعية: | Chromatin, Fluorescence in situ hybridization, Meiosis, Recombination, SIM microscopy, Solanum lycopersicum, Super-resolution |
| الوصف: | Chromatin organization influences gene and transposon expression, and regulates various cellular processes. Higher order chromatin structure has been widely studied using genomic approaches and microscopy image analyses. Chromosome conformation capture and sequencing the junction of DNA fragments enables the study of both chromatin interaction and chromosome folding. However, certain cell types are embedded in other cell types which complicate the process of studying them using high-throughput genomic approaches. To overcome this limitation, high-resolution microscopy techniques are now available to investigate chromatin organization in single cells. In this chapter, we provide a detailed protocol to prepare chromosome spreading from tomato nuclei, to label genomic loci by fluorescence in situ hybridization, and to visualize these locations at high resolution with Structured Illumination microscopy. |
| نوع الوثيقة: | book part |
| اللغة: | unknown |
| العلاقة: | https://doi.org/10.1007/978-1-0716-2253-7_7Test; Lambing, C. 2022. Super-resolution Chromatin Visualization Using a Combined Method of Fluorescence In Situ Hybridization and Structured Illumination Microscopy in Solanum lycopersicum. in: Lambing, C. (ed.) Plant Gametogenesis Springer Nature. pp. 85-92 |
| DOI: | 10.1007/978-1-0716-2253-7_7 |
| الإتاحة: | https://doi.org/10.1007/978-1-0716-2253-7_7Test https://repository.rothamsted.ac.uk/item/98870/super-resolution-chromatin-visualization-using-a-combined-method-of-fluorescence-in-situ-hybridization-and-structured-illumination-microscopy-in-solanum-lycopersicumTest |
| رقم الانضمام: | edsbas.E3C670E2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/978-1-0716-2253-7_7 |
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