دورية أكاديمية
Activation of Sterol-Responsive Element Binding Proteins in a Sucrose-Induced Model of Lysosomal Storage
| العنوان: | Activation of Sterol-Responsive Element Binding Proteins in a Sucrose-Induced Model of Lysosomal Storage |
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| المؤلفون: | Van Beersel, Guillaume, Jadot, Michel, Hamer, Isabelle, Arnould, Thierry |
| المصدر: | Van Beersel , G , Jadot , M , Hamer , I & Arnould , T 2013 , ' Activation of Sterol-Responsive Element Binding Proteins in a Sucrose-Induced Model of Lysosomal Storage ' , The Open Pathology Journal , vol. 7 , 7 , pp. 16-27 . https://doi.org/10.2174/1874375720130905003Test |
| سنة النشر: | 2013 |
| المجموعة: | Research Portal - University of Namur / Portail de la recherche de l'Université de Namur |
| مصطلحات موضوعية: | FASN: fatty acid synthase, HMG-CoA synthase: 3-hydroxy-3-methylglutaryl- Coenzyme A synthase, LDLr: low density lipoprotein receptor, LSDs: lysosomal storage diseases, microtubule-associated protein 1-light chain 3 beta: MAP1-LC3beta/LC3, MVK: mevalonate kinase, SCD-1/2: stearoyl-CoA desaturase 1/2, SREBPs: Sterol Regulatory Element Binding Proteins |
| الوصف: | The accumulation of pinocytosed sucrose in lysosomes constitutes a convenient mean to mimic a lysosomal storage and to study how such storage impacts the biology of the organelle and beyond. Several reports have shown that lysosomal storages can perturb the redistribution of lysosomal lipids, cholesterol in particular. Our goal was to analyse the effect of the intralysosomal accumulation of sucrose on the transcription factors Sterol Regulatory Element Binding Proteins (SREBPs): key actors of the regulation of lipid metabolism. We show that 143B cells grown in the presence of sucrose present a modified distribution of non-esterified cholesterol, which is associated with an increase in the activity of SREBPs. The activation of these transcription factors is associated with an increase in the expression of some of their target genes: HMG-CoA synthase and mevalonate kinase, and with an increase in total lipid biosynthesis. Finally, using siRNA interference we demonstrate that SREBP-2 but not SREBP-1a is responsible for the increase in the expression of the genes encoding these two enzymes. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://researchportal.unamur.be/en/publications/a0fb6eaf-6d2b-41a2-9015-ba6a354f3de3Test |
| DOI: | 10.2174/1874375720130905003 |
| الإتاحة: | https://doi.org/10.2174/1874375720130905003Test https://researchportal.unamur.be/en/publications/a0fb6eaf-6d2b-41a2-9015-ba6a354f3de3Test https://pure.unamur.be/ws/files/11780011/Van_Beersel_et_al._The_Open_Pathology_Journal_2013.pdfTest http://www.benthamscience.com/open/topatj/articles/V007/TOPATJ130905003.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.ADADCFE4 |
| قاعدة البيانات: | BASE |
| DOI: | 10.2174/1874375720130905003 |
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