رسالة جامعية
Participação dos neurônios noradrenérgicos do locus coeruleus nas manifestações comportamentais e respiratórias ao ataque de pânico induzido por CO2 ; The role of locus coeruleus norepinephrine loss on panic-like escape response elicited by exposure to CO2
| العنوان: | Participação dos neurônios noradrenérgicos do locus coeruleus nas manifestações comportamentais e respiratórias ao ataque de pânico induzido por CO2 ; The role of locus coeruleus norepinephrine loss on panic-like escape response elicited by exposure to CO2 |
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| المؤلفون: | Ripamonte, Gabriel Carvalho |
| المساهمون: | Batalhão, Luciane Helena Gargaglioni, http://lattes.cnpq.br/5850453468994497Test, http://lattes.cnpq.br/0751857279960309Test |
| بيانات النشر: | Universidade Federal de São Carlos UFSCar Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF Câmpus São Carlos |
| سنة النشر: | 2022 |
| المجموعة: | Repositório Institucional Universidade Federal de São Carlos (RI UFSCar) |
| مصطلحات موضوعية: | Ataque de pânico, CO2, Noradrenalina, Comportamento, Respiração, Transtorno do pânico, Panic attack, Noradrenaline, Behaviour, Breathing, CIENCIAS BIOLOGICAS::FISIOLOGIA |
| الوصف: | It is well described that imbalances in the modulation of locus coeruleus norepinephrine (LC-NA) neurotransmission are responsible for several psychiatric disorders, including panic disorder. Patients with such a disorder are more sensitive to changes in CO2/pH and therefore are more likely to experience the "suffocation false alarm" which, in turn, triggers the panic attack. LC-NA neurons are involved in ventilatory responses to moderate concentrations of CO2/pH; however, its involvement in behavioral and respiratory responses to severe acidification is still unknown. We investigated the participation of LC-NA neurons by using C56BL/6 mice with a conditional knockout allele of Dbh (DbhcKO) crossed with En1cre (En1CreDbhcKO - LC-NA mutants). In addition, an hypomorphic genotype animal of the same genetic background was used (En1wtDbhcKO) as well as control animals (En1wtDbhwt) of both sexes (10-12 weeks; 20-25 g). The animals went through a period of habituation in ambient air, followed by exposure to normocapnic air (15 min) and then a mixture of gases enriched with 20% CO2 (7 min). The respiratory and metabolic response were VT, fR, VE, VO2 and VE/VO2 to each exposure. The behavioral responses analyzed were escape (jumping/running) and time spent immobile (freezing). In addition, the monoaminergic concentration in the brainstem and forebrain was investigated. A neuroanatomical study to determine which region of the LC communicates with the central nucleus of the amygdaloid complex (CA) and rostral ventrolateral medulla oblonga (RVLM), respectively responsible for fear and anxiety-like behavior and respiratory responses. In these regions, retrobeads were injected and the point of interest was evaluated. In addition, regions in which were activated in LC by 0 and 20% CO2 concentration were also analysed by c-Fos protein immunolocalization. The experimental protocols were approved by the local ethics committee (CEUA - n° 3340/20). LC-NA male mutant and hypomorphic rodents had a hypoventilation and a lower VE/VO2 ... |
| نوع الوثيقة: | thesis |
| اللغة: | Portuguese |
| العلاقة: | RIPAMONTE, Gabriel Carvalho. Participação dos neurônios noradrenérgicos do locus coeruleus nas manifestações comportamentais e respiratórias ao ataque de pânico induzido por CO2. 2022. Dissertação (Mestrado em Ciências Fisiológicas) – Universidade Federal de São Carlos, São Carlos, 2022. Disponível em: https://repositorio.ufscar.br/handle/ufscar/16838Test.; https://repositorio.ufscar.br/handle/ufscar/16838Test |
| الإتاحة: | https://repositorio.ufscar.br/handle/ufscar/16838Test |
| حقوق: | Attribution-NonCommercial-NoDerivs 3.0 Brazil ; http://creativecommons.org/licenses/by-nc-nd/3.0/brTest/ |
| رقم الانضمام: | edsbas.56254AAA |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |