رسالة جامعية
Desenvolvimento de dispositivos descartáveis e de baixo custo para a detecção de biomarcadores relacionados ao câncer de colo uterino e endometrial ; Development of low-cost and disposable devices for the detection of biomarkers related to cervical and endometrial cancer
| العنوان: | Desenvolvimento de dispositivos descartáveis e de baixo custo para a detecção de biomarcadores relacionados ao câncer de colo uterino e endometrial ; Development of low-cost and disposable devices for the detection of biomarkers related to cervical and endometrial cancer |
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| المؤلفون: | Baldo, Thaisa Aparecida |
| المساهمون: | Faria, Ronaldo Censi, http://lattes.cnpq.br/8659496864305621Test, http://lattes.cnpq.br/6150188346082884Test |
| بيانات النشر: | Universidade Federal de São Carlos UFSCar Programa de Pós-Graduação em Química - PPGQ Câmpus São Carlos |
| سنة النشر: | 2019 |
| المجموعة: | Repositório Institucional Universidade Federal de São Carlos (RI UFSCar) |
| مصطلحات موضوعية: | Câncer, Câncer cervical, Câncer endometrial, Diagnóstico precoce, Imunoensaios eletroquímicos, Microfluídica, Biomarcadores proteicos, COX-2, Tp53, p16, E6, Cancer, Cervical cancer, Endometrial cancer, Early diagnosis, Electrochemical immunoassays, Microfluidic, Protein biomarkers, CIENCIAS EXATAS E DA TERRA::QUIMICA, CIENCIAS EXATAS E DA TERRA::QUIMICA::QUIMICA ANALITICA::ELETROANALITICA |
| الوصف: | Cervical cancer (CC) and endometrial cancer (EC) are malignant neoplasms that affect women worldwide and the early diagnosis of these cancers is still limited. The incidence of these malignancies has been increasing year by year and, therefore, there is a great interest in new tools to enable a more accurate early diagnosis, increasing treatment efficiency and, consequently, reducing mortality rates. In this view, this work proposes the construction of disposable electrochemical devices for detection of CC and EC-related protein biomarkers by sandwich-type immunoassays using magnetic particles and nanoparticles for the capture and separation of analytes. These devices were manufactured using screen printing and inkjet printing techniques. The first work developed comprised the construction of screen-printing electrodes, composing a microfluidic cell that was constituted by an arrangement of 8 working electrodes connected with a pseudo-reference electrode and a counter electrode. The device was applied in the multiplexed detection of cyclooxygenase-2 (COX-2) and tumor protein Tp53 (Tp53) biomarkers. These devices were manufactured using screen printing and inkjet printing techniques. The first work developed comprised the construction of screen-printing electrodes, composing a microfluidic cell that was constituted by an arrangement of 8 working electrodes connected with a pseudo-reference electrode and a counter electrode. The device was applied in the multiplexed detection of cyclooxygenase-2 (COX-2) and tumor protein Tp53 (Tp53) biomarkers. For this, monoclonal antibodies (Ab1) were anchored on the surface of previously modified working electrodes with a bilayer formed poly (diallyldimethylammonium chloride) and gold nanoparticles (AuNPs) decorated with glutathione. Magnetic particles (PMs) modified with polyclonal antibodies (Ab2) and enzyme peroxidase (HRP) as electrochemical marker were used to capture and separate the biomarkers in serum samples from patients with CC and CE and injected into the ... |
| نوع الوثيقة: | thesis |
| اللغة: | Portuguese |
| العلاقة: | BALDO, Thaisa Aparecida. Desenvolvimento de dispositivos descartáveis e de baixo custo para a detecção de biomarcadores relacionados ao câncer de colo uterino e endometrial. 2019. Tese (Doutorado em Química) – Universidade Federal de São Carlos, São Carlos, 2019. Disponível em: https://repositorio.ufscar.br/handle/ufscar/11848Test.; https://repositorio.ufscar.br/handle/ufscar/11848Test |
| الإتاحة: | https://repositorio.ufscar.br/handle/ufscar/11848Test |
| حقوق: | Attribution-NonCommercial-NoDerivs 3.0 Brazil ; http://creativecommons.org/licenses/by-nc-nd/3.0/brTest/ |
| رقم الانضمام: | edsbas.84B3E4D4 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |