دورية أكاديمية
UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography
| العنوان: | UV-MALDI-TOF Mass Spectrometry Analysis of Heparin Oligosaccharides Obtained by Nitrous Acid Controlled Degradation and High Performance Anion Exchange Chromatography |
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| المؤلفون: | Bultel, L., Landoni, M., Grand, E., Couto, A.S., Kovensky, J. |
| المصدر: | J. Am. Soc. Mass Spectrom. 2010;21(1):178-190 |
| سنة النشر: | 2010 |
| المجموعة: | Repositorio Digital Institucional - Universidad de Buenos Aires (RDI UBA) |
| مصطلحات موضوعية: | Controlled degradation, Cyano-4-hydroxycinnamic acids, Dihydroxybenzoic acids, Harmane, High-performance anion-exchange chromatography, MALDI-TOF, MALDI-TOF mass spectrometry, Matrix assisted laser desorption/ionization, New tools, Nitrous acid, Structural determination, Sulfate groups, Chromatographic analysis, Chromatography, Degradation, Desorption, Inorganic acids, Mass spectrometry, Oligosaccharides, Polysaccharides, Structural analysis, Ultraviolet lasers, Negative ions, alpha cyano 4 hydroxycinnamic acid, benzoic acid derivative, beta carboline, dihydroxybenzoic acid, heparin, oligosaccharide, sulfate |
| الوصف: | Nitrous acid degradation of heparin followed by high-performance anion-exchange chromatography (HPAEC) separation and ultraviolet matrix assisted laser desorption/ionization time-of-flight (UV-MALDI-TOF) analysis led to the structural determination of six sulfated oligosaccharides. Three different matrices (α-cyano-4-hydroxycinnamic acid (CHCA), nor-harmane, and dihydroxybenzoic acid (DHB)) have been used, and the complementary results obtained allowed in most cases to assign the position of sulfate groups. Based on the different cleavages produced on the purified oligosaccharides in source during the MS analysis by the use of the different matrices, this approach provides a new tool for structural analysis. © 2010 American Society for Mass Spectrometry. ; Fil:Landoni, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Kovensky, J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_BultelTest; http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v21_n1_p178_Bultel_oaiTest |
| الإتاحة: | https://doi.org/20.500.12110/paper_10440305_v21_n1_p178_BultelTest https://hdl.handle.net/20.500.12110/paper_10440305_v21_n1_p178_BultelTest http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_10440305_v21_n1_p178_Bultel_oaiTest |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/2.5/arTest |
| رقم الانضمام: | edsbas.95E5C560 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |