دورية أكاديمية
Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose
| العنوان: | Phase behavior of freeze-dried phospholipid-cholesterol mixtures stabilized with trehalose |
|---|---|
| المؤلفون: | Ohtake, S., Schebor, C., Palecek, S.P., De Pablo, J.J. |
| المصدر: | Biochim. Biophys. Acta Biomembr. 2005;1713(1):57-64 |
| سنة النشر: | 2005 |
| المجموعة: | Repositorio Digital Institucional - Universidad de Buenos Aires (RDI UBA) |
| مصطلحات موضوعية: | Cholesterol, DPPC, DPPE, Freeze-dried, Phase transition, dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine, liposome, phospholipid, trehalose, article, enthalpy, freeze drying, lipid composition, lipid membrane, phase separation, priority journal, temperature sensitivity, thermostability, 1,2-Dipalmitoylphosphatidylcholine, Calorimetry, Differential Scanning, Phosphatidylethanolamines, Mammalia |
| الوصف: | A study is presented of the role of cholesterol content on the gel-to-liquid crystalline phase transition of freeze-dried liposomes stabilized with trehalose, a well known lyoprotectant. The phospholipids considered in this work, DPPC and DPPE, belong to the two predominant phospholipid species found in numerous biological membranes. Cholesterol is found in abundance in mammalian plasma membranes. DSC measurements reveal that cholesterol-containing liposomes exhibit multiple phase transitions upon dehydration. Addition of trehalose to these systems lowers the phase transition temperature and limits the phase separation of the lipidic components upon freeze-drying. This work provides strong evidence for the effectiveness of trehalose in stabilizing cholesterol-containing membranes upon lyophilization. © 2005 Elsevier B.V. All rights reserved. ; Fil:Schebor, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_OhtakeTest; http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00052736_v1713_n1_p57_Ohtake_oaiTest |
| الإتاحة: | https://doi.org/20.500.12110/paper_00052736_v1713_n1_p57_OhtakeTest https://hdl.handle.net/20.500.12110/paper_00052736_v1713_n1_p57_OhtakeTest http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00052736_v1713_n1_p57_Ohtake_oaiTest |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/2.5/arTest |
| رقم الانضمام: | edsbas.A85AFA30 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |