دورية أكاديمية
Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer
| العنوان: | Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer |
|---|---|
| المؤلفون: | Sacca, P., Meiss, R., Casas, G., Mazza, O., Calvo, J.C., Navone, N., Vazquez, E. |
| المصدر: | Br. J. Cancer 2007;97(12):1683-1689 |
| سنة النشر: | 2007 |
| المجموعة: | Repositorio Digital Institucional - Universidad de Buenos Aires (RDI UBA) |
| مصطلحات موضوعية: | Haeme oxygenase-1, Nuclear localisation, Oxidative stress, Prostate cancer, heme oxygenase 1, hemin, adult, aged, article, cancer cell culture, cancer growth, carcinogenesis, cell compartmentalization, cell nucleus, cell protection, controlled study, disease association, disease course, enzyme localization, enzyme regulation, Gleason score, human, human cell, human tissue, immunohistochemistry, male, priority journal, prostate hypertrophy, prostatectomy, protein expression |
| الوصف: | The role of oxidative stress in prostate cancer has been increasingly recognised. Acute and chronic inflammations generate reactive oxygen species that result in damage to cellular structures. Haeme oxygenase-1 (HO-1) has cytoprotective effects against oxidative damage. We hypothesise that modulation of HO-1 expression may be involved in the process of prostate carcinogenesis and prostate cancer progression. We thus studied HO-1 expression and localisation in 85 samples of organ-confined primary prostate cancer obtained via radical prostatectomy (Gleason grades 4-9) and in 39 specimens of benign prostatic hyperplasia (BPH). We assessed HO-1 expression by immunohistochemical staining. No significant difference was observed in the cytoplasmic positive reactivity among tumours (84%), non-neoplastic surrounding parenchyma (89%), or BPH samples (87%) (P=0.53). Haeme oxygenase-1 immunostaining was detected in the nuclei of prostate cancer cells in 55 of 85 (65%) patients but less often in non-neoplastic surrounding parenchyma (30 of 85, 35%) or in BPH (9 of 39, 23%) (P<0.0001). Immunocytochemical and western blot analysis showed HO-1 only in the cytoplasmic compartment of PC3 and LNCaP prostate cancer cell lines. Treatment with hemin, a well-known specific inducer of HO-1, led to clear nuclear localisation of HO-1 in both cell lines and highly induced HO-1 expression in both cellular compartments. These findings have demonstrated, for the first time, that HO-1 expression and nuclear localisation can define a new subgroup of prostate cancer primary tumours and that the modulation of HO-1 expression and its nuclear translocation could represent new avenues for therapy. © 2007 Cancer Research UK. ; Fil:Sacca, P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Calvo, J.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. ; Fil:Vazquez, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | http://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_SaccaTest; http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00070920_v97_n12_p1683_Sacca_oaiTest |
| الإتاحة: | https://doi.org/20.500.12110/paper_00070920_v97_n12_p1683_SaccaTest https://hdl.handle.net/20.500.12110/paper_00070920_v97_n12_p1683_SaccaTest http://repositoriouba.sisbi.uba.ar/gsdl/cgi-bin/library.cgi?a=d&c=artiaex&d=paper_00070920_v97_n12_p1683_Sacca_oaiTest |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/2.5/arTest |
| رقم الانضمام: | edsbas.E561D59C |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |