دورية أكاديمية
Intermittent, low dose carbon monoxide exposure enhances survival and dopaminergic differentiation of human neural stem cells
| العنوان: | Intermittent, low dose carbon monoxide exposure enhances survival and dopaminergic differentiation of human neural stem cells |
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| المؤلفون: | Dreyer-Andersen, Nanna, Almeida, Ana Sofia, Jensen, Pia, Kamand, Morad, Okarmus, Justyna, Rosenberg, Tine, Friis, Stig During, Serrano, Alberto Martinez, Blaabjerg, Morten, Kristensen, Bjarne Winther, Skrydstrup, Troels, Gramsbergen, Jan Bert, Vieira, Helena L. A., Meyer, Morten |
| المساهمون: | NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Centro de Estudos de Doenças Crónicas (CEDOC), Instituto de Tecnologia Química e Biológica António Xavier (ITQB) |
| سنة النشر: | 2018 |
| المجموعة: | Repositório da Universidade Nova de Lisboa (UNL) |
| مصطلحات موضوعية: | HEME OXYGENASE 1, FETAL NIGRAL TRANSPLANTATION, HYPOXIA-INDUCIBLE FACTOR-1, ENDOTHELIAL GROWTH-FACTOR, PARKINSONS-DISEASE, NEURONAL DIFFERENTIATION, ISCHEMIA/REPERFUSION INJURY, INFLAMMATORY RESPONSE, SIGNALING PATHWAYS, MOLECULE-2 CORM-2, SDG 3 - Good Health and Well-being |
| الوصف: | Exploratory studies using human fetal tissue have suggested that intrastriatal transplantation of dopaminergic neurons may become a future treatment for patients with Parkinson’s disease. However, the use of human fetal tissue is compromised by ethical, regulatory and practical concerns. Human stem cells constitute an alternative source of cells for transplantation in Parkinson’s disease, but efficient protocols for controlled dopaminergic differentiation need to be developed. Short-term, low-level carbon monoxide (CO) exposure has been shown to affect signaling in several tissues, resulting in both protection and generation of reactive oxygen species. The present study investigated the effect of CO produced by a novel CO-releasing molecule on dopaminergic differentiation of human neural stem cells. Short-term exposure to 25 ppm CO at days 0 and 4 significantly increased the relative content of β-tubulin III-immunoreactive immature neurons and tyrosine hydroxylase expressing catecholaminergic neurons, as assessed 6 days after differentiation. Also the number of microtubule associated protein 2-positive mature neurons had increased significantly. Moreover, the content of apoptotic cells (Caspase3) was reduced, whereas the expression of a cell proliferation marker (Ki67) was left unchanged. Increased expression of hypoxia inducible factor-1α and production of reactive oxygen species (ROS) in cultures exposed to CO may suggest a mechanism involving mitochondrial alterations and generation of ROS. In conclusion, the present procedure using controlled, short-term CO exposure allows efficient dopaminergic differentiation of human neural stem cells at low cost and may as such be useful for derivation of cells for experimental studies and future development of donor cells for transplantation in Parkinson’s disease. ; publishersversion ; published |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | PURE: 3614988; PURE UUID: 9717c189-3941-463d-a484-3ff902bc9f18; WOS: 000422652700040; PubMed: 29338033; Scopus: 85040556113; ORCID: /0000-0001-9415-3742/work/49897023; http://hdl.handle.net/10362/30670Test; https://doi.org/10.1371/journal.pone.0191207Test |
| DOI: | 10.1371/journal.pone.0191207 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0191207Test http://hdl.handle.net/10362/30670Test |
| حقوق: | openAccess |
| رقم الانضمام: | edsbas.3263D0F5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0191207 |