دورية أكاديمية
Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs
| العنوان: | Blood molecular profile to predict genotoxicity from exposure to antineoplastic drugs |
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| المؤلفون: | Ladeira, Carina, Araújo, Rúben, Ramalhete, Luís, Teixeira, Hélder, Calado, Cecília R.C. |
| المساهمون: | Comprehensive Health Research Centre (CHRC) - Pólo ENSP, Centro de Investigação em Saúde Pública (CISP/PHRC), Escola Nacional de Saúde Pública (ENSP), NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM), Comprehensive Health Research Centre (CHRC) - pólo NMS |
| سنة النشر: | 2023 |
| المجموعة: | Repositório da Universidade Nova de Lisboa (UNL) |
| مصطلحات موضوعية: | Antineoplastics, Cytokinesis-blocked micronucleus assay, Frozen blood, FTIR-spectroscopy, Genotoxicity, Molecular profile, Genetics, Health, Toxicology and Mutagenesis |
| الوصف: | Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL, IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Funding Information: This work was supported by Instituto Politécnico de Lisboa under grant IDI&CA/IPL/2021/PLASCOGEN_ESTeSL , IDI&CA/IPL/2017/GenTox/ESTeSL, and by the Fundação para a Ciência e a Tecnologia, Portugal, under grant DSAIPA/DS/0117/2020. The human biomonitoring had financial support given by Portuguese Authority of Working Conditions (Project reference: 036APJ/09). Publisher Copyright: © 2023 The Authors ; Genotoxicity is an important information that should be included in human biomonitoring programmes. However, the usually applied cytogenetic assays are laborious and time-consuming, reason why it is critical to develop rapid and economic new methods. The aim of this study was to evaluate if the molecular profile of frozen whole blood, acquired by Fourier Transform Infrared (FTIR) spectroscopy, allows to assess genotoxicity in occupational exposure to antineoplastic drugs, as obtained by the cytokinesis-block micronucleus assay. For that purpose, 92 samples of peripheral blood were studied: 46 samples from hospital professionals occupationally exposed to antineoplastic drugs and 46 samples from workers in academia without exposure (controls). It was first evaluated the metabolome from frozen whole blood by methanol precipitation of macromolecules as haemoglobin, followed by centrifugation. The metabolome molecular profile resulted in 3 ratios of spectral bands, significantly different between the exposed and non-exposed group (p < 0.01) and a spectral principal component-linear discriminant analysis (PCA-LDA) model enabling to predict genotoxicity from exposure with 73 % accuracy. After optimization of ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1383-5718 |
| العلاقة: | PURE: 89588447; PURE UUID: 54aa4307-0425-45e2-bb06-01bdf35f93de; Scopus: 85170430854; PubMed: 37770138; WOS: 001076457900001; http://hdl.handle.net/10362/166843Test; https://doi.org/10.1016/j.mrgentox.2023.503681Test |
| DOI: | 10.1016/j.mrgentox.2023.503681 |
| الإتاحة: | https://doi.org/10.1016/j.mrgentox.2023.503681Test http://hdl.handle.net/10362/166843Test |
| حقوق: | openAccess |
| رقم الانضمام: | edsbas.D8F3D4A |
| قاعدة البيانات: | BASE |
| تدمد: | 13835718 |
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| DOI: | 10.1016/j.mrgentox.2023.503681 |