دورية أكاديمية
Development and validation of exhaled breath condensate microRNAs to identify and endotype asthma in children
العنوان: | Development and validation of exhaled breath condensate microRNAs to identify and endotype asthma in children |
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المؤلفون: | Mendes, FC, Paciência, I, Ferreira, AC, Martins, C, Rufo, JC, Silva, D, Cunha, P, Farraia, M, Moreira, P, Delgado, L, Soares, ML, Moreira, A |
المساهمون: | Instituto de Investigação e Inovação em Saúde |
بيانات النشر: | Public Library of Science |
سنة النشر: | 2019 |
المجموعة: | Repositório Aberto da Universidade do Porto |
مصطلحات موضوعية: | Adolescent, Asthma / diagnosis, Asthma / genetics, Asthma / physiopathology, Biomarkers, Breath Tests, Child, Exhalation, Female, Genetic Association Studies, Genetic Predisposition to Disease, Humans, Male, MicroRNAs / genetics, Nitric Oxide / analysis, Phenotype, Surveys and Questionnaires |
الوصف: | Detection and quantification of microRNAs (miRNAs) in exhaled breath condensate (EBC) has been poorly explored. Therefore we aimed to assess miRNAs in EBC as potential biomarkers to diagnose and endotype asthma in school aged children. In a cross sectional, nested case control study, all the asthmatic children (n = 71) and a random sample of controls (n = 115), aged 7 to 12 years, attending 71 classrooms from 20 local schools were selected and arbitrarily allocated to the development or validation set. Participants underwent skin-prick testing, spirometry with bronchodilation, had exhaled level of nitric oxide determined and EBC collected. Based on previous studies eleven miRNAs were chosen and analyzed in EBC by reverse transcription-quantitative real-time PCR. Principal component analysis was applied to identify miRNAs profiles and associations were estimated using regression models. In the development set (n = 89) two clusters of miRNAs were identified. After adjustments, cluster 1 and three of its clustered miRNAs, miR-126-3p, miR-133a-3p and miR-145-5p were positively associated with asthma. Moreover miR-21-5p was negatively associated with symptomatic asthma and positively associated with positive bronchodilation without symptoms. An association was also found between miR-126-3p, cluster 2 and one of its clustered miRNA, miR-146-5p, with higher FEF25-75 reversibility. These findings were confirmed in the validation set (n = 97) where two identical clusters of miRNAs were identified. Additional significant associations were observed between miR-155-5p with symptomatic asthma, negative bronchodilation with symptoms and positive bronchodilation without symptoms. We showed that microRNAs can be measured in EBC of children and may be used as potential biomarkers of asthma, assisting asthma endotype establishment. ; Authors gratefully acknowledge the funding by Fundação para a Ciência e Tecnologia through the Project NORTE-01-0145-FEDER-000010 - Health, Comfort and Energy in the Built Environment (HEBE), ... |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 1932-6203 |
العلاقة: | PLoS ONE, vol.14(11):e0224983; https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0224983Test; https://hdl.handle.net/10216/139010Test |
DOI: | 10.1371/journal.pone.0224983 |
الإتاحة: | https://doi.org/10.1371/journal.pone.0224983Test https://hdl.handle.net/10216/139010Test |
حقوق: | info:eu-repo/semantics/openAccess |
رقم الانضمام: | edsbas.C46E5494 |
قاعدة البيانات: | BASE |
تدمد: | 19326203 |
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DOI: | 10.1371/journal.pone.0224983 |