دورية أكاديمية
Mechanisms of liver fibrosis associated with experimental fasciola hepatica infection: Roles of Fas2 proteinase and hepatic stellate cell activation
| العنوان: | Mechanisms of liver fibrosis associated with experimental fasciola hepatica infection: Roles of Fas2 proteinase and hepatic stellate cell activation |
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| المؤلفون: | Marcos, L.A., Terashima, A., Yi, P., Andrade, R., Cubero, F.J., Albanis, E., Gotuzzo, E., Espinoza, J.R., Friedman, S.L. |
| بيانات النشر: | American Society of Parasitologists |
| سنة النشر: | 2011 |
| المجموعة: | Repositorio - UPCH (Universidad Peruana Cayetano Heredia) |
| مصطلحات موضوعية: | Humans|controlled study, pathology, animal, animal experiment, animal model, animal tissue, Animals, collagen type 1, genetics, in vitro study, in vivo study, metabolism, non|physiology, pathogenicity, polymerase chain reaction, infectious disease, parasite antigen, unclassified drug, disease association, gene expression, Disease Models, Analysis of Variance, Cell Line, messenger RNA, upregulation, disease model, real time polymerase chain reaction, enzymology, tissue inhibitor of metalloproteinase 1, Fasciola hepatica |
| الوصف: | We have evaluated the possible mechanisms of liver fibrosis caused by Fasciola hepatica in an animal model and in culture using immortalized human stellate cells. Liver biopsies of F. hepatica-infected rats were performed at wk 8 and 16. Serum-starved LX-2 cells, a human stellate cell line, were exposed to increasing concentrations of Fas2 antigen. The expression of key fibrosis-related genes was evaluated by qRT-PCR. There was a significant correlation between fibrogenic gene expression and both intensity and duration of infection. LX-2 cells exposed to Fas2 showed progressively increased expression of mRNAs for Collagen I, alpha-smooth muscle-actin, platelet-derived growth factor beta receptor, and tissue inhibitor of metalloproteinase II; inhibition of Fas2 cysteine proteinase activity by E-64 abrogated these increases, suggesting that the protease activity of Fas2 is involved in fibrogenic stimulation. In summary, F. hepatica infection is associated with up-regulation of mRNAs associated with hepatic fibrogenesis in vivo and in activated hepatic stellate cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1937-2345 |
| العلاقة: | urn:issn:1937-2345; https://hdl.handle.net/20.500.12866/11191Test; Journal of Parasitology; https://doi.org/10.1645/GE-2420.1Test |
| DOI: | 10.1645/GE-2420.1 |
| الإتاحة: | https://doi.org/20.500.12866/11191Test https://doi.org/10.1645/GE-2420.1Test https://hdl.handle.net/20.500.12866/11191Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-nd/4.0/deed.esTest |
| رقم الانضمام: | edsbas.D649E971 |
| قاعدة البيانات: | BASE |
| تدمد: | 19372345 |
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| DOI: | 10.1645/GE-2420.1 |