دورية أكاديمية
Rapid Diagnostic Tests for Malaria Diagnosis in the Peruvian Amazon: Impact of pfhrp2 Gene Deletions and Cross-Reactions
| العنوان: | Rapid Diagnostic Tests for Malaria Diagnosis in the Peruvian Amazon: Impact of pfhrp2 Gene Deletions and Cross-Reactions |
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| المؤلفون: | Maltha, J., Gamboa, D., Bendezu, J., Sanchez, L., Cnops, L., Gillet, P., Jacobs, J. |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2012 |
| المجموعة: | Repositorio - UPCH (Universidad Peruana Cayetano Heredia) |
| مصطلحات موضوعية: | Humans, Peru, major clinical study, diagnostic test, gene deletion, Malaria, Plasmodium vivax, Plasmodium falciparum, rapid diagnostic test, Polymerase Chain Reaction, gene, Geography, Models, Genetic, Microscopy, real time polymerase chain reaction, Prospective Studies, malaria falciparum, Plasmodium vivax malaria, Protozoan Proteins, mixed infection, enzyme linked immunosorbent assay, DNA extraction, diagnostic accuracy, diagnostic procedure, Antigens, Protozoan, sensitivity analysis, cross reaction, lactate dehydrogenase |
| الوصف: | Background: In the Peruvian Amazon, Plasmodium falciparum and Plasmodium vivax malaria are endemic in rural areas, where microscopy is not available. Malaria rapid diagnostic tests (RDTs) provide quick and accurate diagnosis. However, pfhrp2 gene deletions may limit the use of histidine-rich protein-2 (PfHRP2) detecting RDTs. Further, cross-reactions of P. falciparum with P. vivax-specific test lines and vice versa may impair diagnostic specificity. Methods: Thirteen RDT products were evaluated on 179 prospectively collected malaria positive samples. Species diagnosis was performed by microscopy and confirmed by PCR. Pfhrp2 gene deletions were assessed by PCR. Results: Sensitivity for P. falciparum diagnosis was lower for PfHRP2 compared to P. falciparum-specific Plasmodium lactate dehydrogenase (Pf-pLDH)- detecting RDTs (71.6% vs. 98.7%, p<0.001). Most (19/21) false negative PfHRP2 results were associated with pfhrp2 gene deletions (25.7% of 74 P. falciparum samples). Diagnostic sensitivity for P. vivax (101 samples) was excellent, except for two products. In 10/12 P. vivax-detecting RDT products, cross-reactions with the PfHRP2 or Pf-pLDH line occurred at a median frequency of 2.5% (range 0%-10.9%) of P. vivax samples assessed. In two RDT products, two and one P. falciparum samples respectively cross-reacted with the Pv-pLDH line. Two Pf-pLDH/pan-pLDH-detecting RDTs showed excellent sensitivity with few (1.0%) cross-reactions but showed faint Pf-pLDH lines in 24.7% and 38.9% of P. falciparum samples. Conclusion: PfHRP2-detecting RDTs are not suitable in the Peruvian Amazon due to pfhrp2 gene deletions. Two Pf-pLDH-detecting RDTs performed excellently and are promising RDTs for this region although faint test lines are of concern. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | urn:issn:1932-6203; https://hdl.handle.net/20.500.12866/11102Test; PLoS ONE; https://doi.org/10.1371/journal.pone.0043094Test |
| DOI: | 10.1371/journal.pone.0043094 |
| الإتاحة: | https://doi.org/20.500.12866/11102Test https://doi.org/10.1371/journal.pone.0043094Test https://hdl.handle.net/20.500.12866/11102Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-nd/4.0/deed.esTest |
| رقم الانضمام: | edsbas.9E820382 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0043094 |