دورية أكاديمية
Molecular architecture of the human GINS complex.
| العنوان: | Molecular architecture of the human GINS complex. |
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| المؤلفون: | Boskovic, Jasminka, Coloma, Javier, Aparicio, Tomás, Zhou, Min, Robinson, Carol V, Mendez, Juan, Montoya, Guillermo |
| المساهمون: | Fundación Caja Madrid, Ministerio de Educación y Ciencia (España) |
| بيانات النشر: | EMBO Press |
| سنة النشر: | 2007 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | Cell Cycle Proteins, DNA Helicases, DNA Replication, DNA-Binding Proteins, Humans, Minichromosome Maintenance Complex Component 2, Minichromosome Maintenance Complex Component 7, Models, Molecular, Nuclear Proteins, Protein Binding, Protein Conformation |
| الوصف: | Chromosomal DNA replication is strictly regulated through a sequence of steps that involve many macromolecular protein complexes. One of these is the GINS complex, which is required for initiation and elongation phases in eukaryotic DNA replication. The GINS complex consists of four paralogous subunits. At the G1/S transition, GINS is recruited to the origins of replication where it assembles with cell-division cycle protein (Cdc)45 and the minichromosome maintenance mutant (MCM)2-7 to form the Cdc45/Mcm2-7/GINS (CMG) complex, the presumed replicative helicase. We isolated the human GINS complex and have shown that it can bind to DNA. By using single-particle electron microscopy and three-dimensional reconstruction, we obtained a medium-resolution volume of the human GINS complex, which shows a horseshoe shape. Analysis of the protein interactions using mass spectrometry and monoclonal antibody mapping shows the subunit organization within the GINS complex. The structure and DNA-binding data suggest how GINS could interact with DNA and also its possible role in the CMG helicase complex. ; We thank O. LLorca and J.M. Valpuesta for the use of the electronmicroscopy and for helpful comments, and J. Prieto for advice with theanalytical ultracentrifugue. J.B. and J.C. thank the Ministerio deEducacio ́n y Ciencia for a Ramo ́n y Cajal contract and a pre-doctoralfellowship. Funding was obtained through MEC grants BFU2005-02403,GEN2003-20642-C09-02 to G.M., and European Union 3D-RepertoireLSHG-CT-2005-512028 to G.M., M.Z. and C.V.R., and MEC BFU2004-04886 and Fundacio ́n Caja Madrid to J.M ; Sí |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1469-221X |
| العلاقة: | https://doi.orgTest/ 10.1038/sj.embor.7401002.; EMBO Rep . 2007 ;8(7):678-84.; http://hdl.handle.net/20.500.12105/17665Test; EMBO reports |
| DOI: | 10.1038/sj.embor.7401002 |
| الإتاحة: | https://doi.org/20.500.12105/17665Test https://doi.org/10.1038/sj.embor.7401002Test https://hdl.handle.net/20.500.12105/17665Test |
| حقوق: | http://creativecommons.org/licenses/by-nc-nd/4.0Test/ ; Attribution-NonCommercial-NoDerivatives 4.0 Internacional ; open access |
| رقم الانضمام: | edsbas.BA4DD8EB |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1469221X |
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| DOI: | 10.1038/sj.embor.7401002 |