دورية أكاديمية
Behavioural immune landscapes of inflammation.
| العنوان: | Behavioural immune landscapes of inflammation. |
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| المؤلفون: | Crainiciuc, Georgiana, Palomino-Segura, Miguel, Molina-Moreno, Miguel, Sicilia, Jon, Aragones, David G, Li, Jackson Liang Yao, Madurga, Rodrigo, Adrover, Jose M, Aroca-Crevillen, Alejandra, Martin-Salamanca, Sandra, Del Valle, Alfonso Serrano, Castillo, Sandra D, Welch, Heidi C E, Soehnlein, Oliver, Graupera, Mariona, Sanchez-Cabo, Fatima, Zarbock, Alexander, Smithgall, Thomas E, Di Pilato, Mauro, Mempel, Thorsten R, Tharaux, Pierre-Louis, González, Santiago F, Ayuso-Sacido, Angel, Ng, Lai Guan, Calvo, Gabriel F, González-Díaz, Iván, Díaz-de-María, Fernando, Hidalgo, Andres |
| المساهمون: | Ministerio de Ciencia e Innovación (España), Fundación La Caixa, Transatlantic Network of Excellence, Fondation Leducq, Unión Europea. Comisión Europea, Federation of European Biochemical Societies, European Molecular Biology Organization, Fundación ProCNIC, Marie Curie |
| بيانات النشر: | Nature Publishing Group |
| سنة النشر: | 2022 |
| المجموعة: | REPISALUD (REPositorio Institucional en SALUD del Instituto de Salud Carlos III - ISCIII) |
| مصطلحات موضوعية: | Inflammation, Leukocytes, Proteomics, Animals, Cell Shape, Endothelium, Mice, Neutrophils, Proto-Oncogene Proteins, src-Family Kinases |
| الوصف: | Transcriptional and proteomic profiling of individual cells have revolutionized interpretation of biological phenomena by providing cellular landscapes of healthy and diseased tissues1,2. These approaches, however, do not describe dynamic scenarios in which cells continuously change their biochemical properties and downstream 'behavioural' outputs3-5. Here we used 4D live imaging to record tens to hundreds of morpho-kinetic parameters describing the dynamics of individual leukocytes at sites of active inflammation. By analysing more than 100,000 reconstructions of cell shapes and tracks over time, we obtained behavioural descriptors of individual cells and used these high-dimensional datasets to build behavioural landscapes. These landscapes recognized leukocyte identities in the inflamed skin and trachea, and uncovered a continuum of neutrophil states inside blood vessels, including a large, sessile state that was embraced by the underlying endothelium and associated with pathogenic inflammation. Behavioural screening in 24 mouse mutants identified the kinase Fgr as a driver of this pathogenic state, and interference with Fgr protected mice from inflammatory injury. Thus, behavioural landscapes report distinct properties of dynamic environments at high cellular resolution. ; This study was supported by RTI2018-095497-B-I00 from Ministerio de Ciencia e Innovación (MCIN), HR17_00527 from Fundación La Caixa, Transatlantic Network of Excellence (TNE-18CVD04) from the Leducq Foundation, and FET-OPEN (no. 861878) from the European Commission to A.H. M.P-S. is supported by a Federation of European Biochemical Societies and the EMBO ALTF (no. 1142-2020) long-term fellowships. J.S. is supported by a fellowship (PRE2019-089130) from MICINN and A.A.-C. is supported by fellowship CF/BQ/ DR19/11740022 from La Caixa Foundation. J.L.Y.L. was supported by A*STAR and a Juan de la Cierva JCI-2017-33136 Fellowship from MICINN. S.D.C. is a recipient of a Marie Sklodowska-Curie fellowship (749731). M.G. is supported by ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1476-4687 |
| العلاقة: | info:eu-repo/grantAgreement/ES/RTI2018-095497-B-I00; info:eu-repo/grantAgreement/ES/HR17_00527; info:eu-repo/grantAgreement/ES/MCIN/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/ES/PID2019-110895RB-I00; info:eu-repo/grantAgreement/ES/SBPLY/19/180501/000211; info:eu-repo/grantAgreement/ES/RTI2018-102084-B-I00; Nature. 2022 Jan;601(7893):415-421; http://hdl.handle.net/20.500.12105/15656Test; Nature |
| DOI: | 10.1038/s41586-021-04263-y |
| الإتاحة: | https://doi.org/20.500.12105/15656Test https://doi.org/10.1038/s41586-021-04263-yTest https://hdl.handle.net/20.500.12105/15656Test |
| حقوق: | http://creativecommons.org/licenses/by/4.0Test/ ; Atribución 4.0 Internacional ; open access |
| رقم الانضمام: | edsbas.A2601219 |
| قاعدة البيانات: | BASE |
| تدمد: | 14764687 |
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| DOI: | 10.1038/s41586-021-04263-y |