دورية أكاديمية
Characterization of an Aux/IAA cDNA upregulated in Pinus pinaster roots in response to colonization by the ectomycorrhizal fungus Hebeloma cylindrosporum.
العنوان: | Characterization of an Aux/IAA cDNA upregulated in Pinus pinaster roots in response to colonization by the ectomycorrhizal fungus Hebeloma cylindrosporum. |
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المؤلفون: | Charvet-Candela, V., Hitchin, S., Ernst, D., Sandermann, H., Marmeisse, R., Gay, G. |
المصدر: | New Phytol. 154, 769-777 (2002) |
بيانات النشر: | Blackwell Publication |
سنة النشر: | 2002 |
المجموعة: | PuSH - Publikationsserver des Helmholtz Zentrums München |
مصطلحات موضوعية: | auxin-regulated gene, Aux/IAA, Hebeloma cylindrosporum, mutant, mycorrhiza, Pinus pinaster |
الوصف: | In an attempt to determine whether fungal auxin affects host plant gene expression during mycorrhizal formation, an auxin upregulated cDNA, Pp-iaa88, was isolated by differential screening of a cDNA library made from auxin-treated Pinus pinaster roots.Pp-iaa88 codes for a polypeptide that shares extensive homology to auxin-inducible Aux/IAA proteins, which are supposed to act as transcription factors. Cycloheximide did not inhibit auxin-induced mRNA accumulation, indicating that Pp-iaa88 upregulation is a primary (direct) auxin response.The level of Pp-iaa88 transcripts in roots increased following inoculation with either an indoleacetic acid-overproducing mutant or a wild-type strain of the ectomycorrhizal fungus Hebeloma cylindrosporum. With both strains, mRNA accumulation was detectable as soon as fungal hyphae reached the root and it increased during differentiation of symbiotic structures. The kinetics of Pp-iaa88 transcript accumulation was closely connected with the dynamics of symbiosis establishment and was more rapid with the mutant than with the wild-type strain.As a putative transcription factor expressed at the very early stages of symbiosis establishment, Pp-iaa88 could play a key role in mycorrhizal formation. |
نوع الوثيقة: | article in journal/newspaper |
اللغة: | English |
تدمد: | 0028-646X 1469-8137 |
العلاقة: | info:eu-repo/semantics/altIdentifier/wos/undefined; info:eu-repo/semantics/altIdentifier/isbn/0028-646X; info:eu-repo/semantics/altIdentifier/pissn/0028-646X; info:eu-repo/semantics/altIdentifier/eissn/1469-8137; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=9974Test; urn:isbn:0028-646X; urn:issn:0028-646X; urn:issn:1469-8137 |
الإتاحة: | https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=9974Test |
حقوق: | info:eu-repo/semantics/closedAccess |
رقم الانضمام: | edsbas.4CE50092 |
قاعدة البيانات: | BASE |
تدمد: | 0028646X 14698137 |
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