دورية أكاديمية
Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6J) tissues.
| العنوان: | Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6J) tissues. |
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| المؤلفون: | Nagler, J., Schriever, S.C., de Angelis, M., Pfluger, P.T., Schramm, K.-W. |
| المصدر: | Biomed. Chromatogr. 32:e4151 (2018) |
| بيانات النشر: | Wiley |
| سنة النشر: | 2018 |
| المجموعة: | PuSH - Publikationsserver des Helmholtz Zentrums München |
| مصطلحات موضوعية: | Ecd, Electrochemical Detection, High-performance Liquid Chromatography, Neurotransmitters, Peripheral Tissues |
| الوصف: | Monoamines, acting as hormones and neurotransmitters, play a critical role in multiple physiological processes ranging from cognitive function and mood to sympathetic nervous system activity, fight-or-flight response and glucose homeostasis. In addition to brain and blood, monoamines are abundant in several tissues, and dysfunction in their synthesis or signaling is associated with various pathological conditions. It was our goal to develop a method to detect these compounds in peripheral murine tissues. In this study, we employed a high-performance liquid chromatography method using electrochemical detection that allows not only detection of catecholamines but also a detailed analysis of nine monoamines and metabolites in murine tissues. Simple tissue extraction procedures were optimized for muscle (gastrocnemius, extensor digitorum longus and soleus), liver, pancreas and white adipose tissue in the range of weight 10-200mg. The system allowed a limit of detection between 0.625 and 2.5pg L-1 for monoamine analytes and their metabolites, including dopamine, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, homovanillic acid, norepinephrine, epinephrine, 3-methoxy-4-hydroxyphenylglycol, serotonin and 5-hydroxyindoleacetic acid. Typical concentrations for different monoamines and their metabolization products in these tissues are presented for C57Bl/6J mice fed a high-fat diet. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | German |
| تدمد: | 0269-3879 1099-0801 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/29166705; info:eu-repo/semantics/altIdentifier/wos/WOS:000427236200017; info:eu-repo/semantics/altIdentifier/isbn/0269-3879; info:eu-repo/semantics/altIdenti; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=52395Test; urn:isbn:0269-3879; urn:issn:0269-3879; urn:issn:1099-0801 |
| DOI: | 10.1002/bmc.4151 |
| الإتاحة: | https://doi.org/10.1002/bmc.4151Test https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=52395Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3AAA8057 |
| قاعدة البيانات: | BASE |
| تدمد: | 02693879 10990801 |
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| DOI: | 10.1002/bmc.4151 |