دورية أكاديمية
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Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.

التفاصيل البيبلوغرافية
العنوان: Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes.
المؤلفون: Klionsky, D.J., Abeliovich, H., Agostinis, P., Agrawal, D.K., Aliev, G., Askew, D.S., Baba, M., Baehrecke, E.H., Bahr, BA., Ballabio, A., Bamber, BA., Bassham, D.C., Bergamini, E., Bi, X., Biard-Piechaczyk, M., Blum, J.S., Bredesen, D.E., Brodsky, J.L., Brumell, J.H., Brunk, U.T., Bursch, W., Camougrand, N., Cebollero, E., Cecconi, F., Chen, Y., Chin, L.S., Choi, A., Chu, C.T., Chung, J., Clarke, P.G., Clark, R.S., Clarke, S.G., Clavé, C., Cleveland, J.L., Codogno, P., Colombo, M.I., Coto-Montes, A., Cregg, J.M., Cuervo, A.M., Debnath, J., Demarchi, F., Dennis, P.B., Dennis, P.A., Deretic, V., Devenish, R.J., Di, Sano, F., Dice, J.F., Difiglia, M., Dinesh-Kumar, S., Distelhorst, C.W., Djavaheri-Mergny, M., Dorsey, F.C., Dröge, W., Dron, M., Dunn, W.A, Jr., Duszenko, M., Eissa, N.T., Elazar, Z., Esclatine, A., Eskelinen, E.L., Fésüs, L., Finley, KD., Fuentes, J.M., Fueyo, J., Fujisaki, K., Galliot, B., Gao, F.B., Gewirtz, D.A., Gibson, SB., Gohla, A., Goldberg, A.L., González, R., González-Estévez, C., Gorski, S., Gottlieb, R.A., Häussinger, D., He, Y.W., Heidenreich, K., Hill, J.A., Høyer-Hansen, M., Hu, X., Huang, W.P., Iwasaki, A., Jäättelä, M., Jackson, W.T., Jiang, X., Jin, S., Johansen, T., Jung, J.U., Kadowaki, M., Kang, C., Kelekar, A., Kessel, D.H., Kiel, J.A., Kim, H.P., Kimchi, A., Kinsella, T.J., Kiselyov, K., Kitamoto, K., Knecht, E., Komatsu, M., Kominami, E., Kondo, S., Kovács, A.L., Kroemer, G., Kuan, C.Y., Kumar, R., Kundu, M., Landry, J., Laporte, M., Le, W., Lei, H.Y., Lenardo, M.J., Levine, B., Lieberman, A., Lim, K.L., Lin, F.C., Liou, W., Liu, L.F., Lopez-Berestein, G., López-Otín, C., Lu, B., Macleod, K.F., Malorni, W., Martinet, W., Matsuoka, K., Mautner, J., Meijer, A.J., Meléndez, A., Michels, P., Miotto, G., Mistiaen, W.P., Mizushima, N., Mograbi, B., Monastyrska, I., Moore, M.N., Moreira, P.I., Moriyasu, Y., Motyl, T., Münz, C., Murphy, L.O., Naqvi, N.I., Neufeld, T.P., Nishino, I., Nixon, R.A., Noda, T., Nürnberg, B., Ogawa, M., Oleinick, NL., Olsen, LJ., Ozpolat, B., Paglin, S., Palmer, G.E., Papassideri, I., Parkes, M., Perlmutter, D.H., Perry, G., Piacentini, M., Pinkas-Kramarski, R., Prescott, M., Proikas-Cezanne, T., Raben, N., Rami, A., Reggiori, F., Rohrer, B., Rubinsztein, D.C., Ryan, K.M., Sadoshima, J., Sakagami, H., Sakai, Y., Sandri, M., Sasakawa, C., Sass, M., Schneider, C., Seglen, P.O., Seiliez, I., Seleverstov, O., Settleman, J., Shacka, J.J., Shapiro, I.M., Sibirny, A., Silva-Zacarin, E.C., Simon, H.U., Simone, C., Simonsen, A., Smith, M.A., Spanel-Borowski, K., Srinivas, V., Steeves, M., Stenmark, H., Stromhaug, P.E., Subauste, C.S., Sugimoto, S., Sulzer, D., Suzuki, T., Swanson, M.S., Tabas, I., Takeshita, F., Talbot, N.J., Tallóczy, Z., Tanaka, K., Tanida, I., Taylor, G.S., Taylor, J.P., Terman, A., Tettamanti, G., Thompson, C.B., Thumm, M., Tolkovsky, A.M., Tooze, S.A., Truant, R., Tumanovska, L.V., Uchiyama, Y., Ueno, T., Uzcátegui, N.L., van der Klei, I., Vaquero, E.C., Vellai, T., Vogel, M.W., Wang, H.G., Webster, P., Wiley, J.W., Xi, Z., Xiao, G., Yahalom, J., Yang, J.M., Yap, G., Yin, X.M., Yoshimori, T., Yu, L., Yue, Z., Yuzaki, M., Zabirnyk, O., Zheng, X., Zhu, X., Deter, R.L.
المصدر: Autophagy 4, 151-175 (2008)
بيانات النشر: Landes Bioscience
سنة النشر: 2008
المجموعة: PuSH - Publikationsserver des Helmholtz Zentrums München
مصطلحات موضوعية: autolysosome, autophagosome, flux, lysosome, phagophore, stress, vacuole
الوصف: Research in autophagy continues to accelerate,(1) and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.(2,3) There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.
نوع الوثيقة: article in journal/newspaper
وصف الملف: application/pdf
اللغة: English
تدمد: 1554-8627
1554-8635
العلاقة: info:eu-repo/semantics/altIdentifier/pmid/18188003; info:eu-repo/semantics/altIdentifier/wos/000253008800003; info:eu-repo/semantics/altIdentifier/isbn/1554-8627; info:eu-repo/semantics/altIdentifier/pissn/1554-8627; info:eu-repo/semantics/altIdentifier/eiss; https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=163Test; urn:isbn:1554-8627; urn:issn:1554-8627; urn:issn:1554-8635
الإتاحة: https://push-zb.helmholtz-muenchen.de/frontdoor.php?source_opus=163Test
حقوق: info:eu-repo/semantics/closedAccess
رقم الانضمام: edsbas.D9C355D
قاعدة البيانات: BASE
الوصف
تدمد:15548627
15548635