دورية أكاديمية
SLC25A19 is required for NADH homeostasis and mitochondrial respiration.
| العنوان: | SLC25A19 is required for NADH homeostasis and mitochondrial respiration. |
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| المؤلفون: | Jiang, Zongsheng |
| المصدر: | Free Radic Biol Med ; ISSN:1873-4596 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Electron transport chain, Idebenone, Mitochondrial respiration, Mitochondrial transporter, NADH, SLC25A19, TPP |
| الوصف: | Mitochondrial transporters facilitate the translocation of metabolites between the cytoplasm and mitochondria and are critical for mitochondrial functional integrity. Although many mitochondrial transporters are associated with metabolic diseases, how they regulate mitochondrial function and their metabolic contributions at the cellular level are largely unknown. Here, we show that mitochondrial thiamine pyrophosphate (TPP) transporter SLC25A19 is required for mitochondrial respiration. SLC25A19 deficiency leads to reduced cell viability, increased integrated stress response (ISR), enhanced glycolysis and elevated cell sensitivity to 2-deoxyglucose (2-DG) treatment. Through a series of biochemical assays, we found that the depletion of mitochondrial NADH is the primary cause of the impaired mitochondrial respiration in SLC25A19 deficient cells. We also showed involvement of SLC25A19 in regulating the enzymatic activities of complexes I and III, the tricarboxylic acid (TCA) cycle, malate-aspartate shuttle and amino acid metabolism. Consistently, addition of idebenone, an analog of coenzyme Q10, restores mitochondrial respiration and cell viability in SLC25A19 deficient cells. Together, our findings provide new insight into the functions of SLC25A19 in mitochondrial and cellular physiology, and suggest that restoring mitochondrial respiration could be a novel strategy for treating SLC25A19-associated disorders. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.freeradbiomed.2024.06.019Test; https://pubmed.ncbi.nlm.nih.gov/38944213Test |
| DOI: | 10.1016/j.freeradbiomed.2024.06.019 |
| الإتاحة: | https://doi.org/10.1016/j.freeradbiomed.2024.06.019Test https://pubmed.ncbi.nlm.nih.gov/38944213Test |
| حقوق: | Copyright © 2024. Published by Elsevier Inc. |
| رقم الانضمام: | edsbas.220AF04A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.freeradbiomed.2024.06.019 |
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