دورية أكاديمية
Polyphenol Composition, Antioxidant Capacity and Xanthine Oxidase Inhibition Mechanism of Furong Plum Fruits at Different Maturity Stages.
| العنوان: | Polyphenol Composition, Antioxidant Capacity and Xanthine Oxidase Inhibition Mechanism of Furong Plum Fruits at Different Maturity Stages. |
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| المؤلفون: | Zheng, Zhipeng, Wu, Li, Deng, Wei, Yi, Kexin, Li, Yibin |
| المصدر: | Foods ; ISSN:2304-8158 ; Volume:12 ; Issue:23 |
| بيانات النشر: | MDPI |
| سنة النشر: | 2023 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Furong plum, antioxidant, inhibition kinetics, polyphenols, xanthine oxidase |
| الوصف: | An experiment was conducted on the polyphenol content, flavonoid content, anthocyanin content, and antioxidant capacity of Furong plum (Prunus salicina Lindl. cv. "furong") at different maturity stages to determine the most suitable maturity stage. The inhibition of plum polyphenols on xanthine oxidase (XOD) was measured, and its kinetics were studied to reveal the inhibitory mechanism. The experimental results showed that the polyphenol, flavonoid and anthocyanin contents of plums at the ripe stage were the highest, reaching 320.46 mg GAE/100 g FW, 204.21 mg/100 g FW, and 66.24 mg/100 g FW, respectively, in comparison those of the plums at the immature and mid-ripe stages. The antioxidant capacity of the ripe plums was stronger than it was during the other stages of the plums growth. Among them, the total polyphenols of the ripe plums exhibited the strongest antioxidant capacity (IC50 values against DPPH and hydroxyl radicals were 28.19 ± 0.67 μg/mL and 198.16 ± 7.55 μg/mL, respectively), which was between the antioxidant capacity of the free polyphenols and bound polyphenols. The major phenolic monomer compounds of plum polyphenols were flavan-3-ols (epicatechin, catechin, proanthocyanidin, and procyanidin B2), flavonols (myricetin), and phenolic acids (chlorogenic acid, ferulic acid, and protocatechuic acid). Additionally, plum polyphenols exhibited a strong inhibitory effect on XOD, with an IC50 value of 77.64 μg/mL. The inhibition kinetics showed that plum polyphenols are mixed-type inhibitors that inhibit XOD activity and that the inhibition process is reversible. The calculated values of Ki and α were 16.53 mmol/L and 0.26, respectively. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.3390/foods12234253Test; https://pubmed.ncbi.nlm.nih.gov/38231765Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10705914Test/ |
| DOI: | 10.3390/foods12234253 |
| الإتاحة: | https://doi.org/10.3390/foods12234253Test https://pubmed.ncbi.nlm.nih.gov/38231765Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10705914Test/ |
| رقم الانضمام: | edsbas.6DDADDB2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3390/foods12234253 |
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