دورية أكاديمية
Ultrasensitive Fluorescent Microsensors Based on Aptamers Modified with SYBR Green I for Visual Quantitative Detection of Organophosphate Pesticides.
| العنوان: | Ultrasensitive Fluorescent Microsensors Based on Aptamers Modified with SYBR Green I for Visual Quantitative Detection of Organophosphate Pesticides. |
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| المؤلفون: | Zhang, Qianru, Liu, Anqi, Song, Xin, Xu, Shihao, Da, Liangguo, Lin, Dan, Jiang, Changlong |
| المصدر: | Anal Chem ; ISSN:1520-6882 ; Volume:96 ; Issue:23 |
| بيانات النشر: | American Chemical Society |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| الوصف: | Organophosphate pesticides (OPs) are widely utilized in agricultural production, and the residues threaten public health and environmental safety due to their toxicity. Herein, a novel and simple DNA aptamer-based sensor has been fabricated for the rapid, visual, and quantitative detection of profenofos and isocarbophos. The proposed DNA aptamers with a G-quadruplex spatial structure could be recognized by SYBR Green I (SG-I), resulting in strong green fluorescence emitted by SG-I. The DNA aptamers exhibit a higher specific binding ability to target OP molecules through aromatic ring stacking, disrupting the interaction between SG-I and DNA aptamers to induce green fluorescence quenching. Meanwhile, the fluorescence wavelength of G-quadruplex fluorescence emission peaks changes, accompanied by an obvious fluorescence variation from green to blue. SG-I-modified aptasensor without any additive reference fluorescence units for use in multicolor fluorescence assay for selective monitoring of OPs was first developed. The developed aptasensor provides a favorable linear range from 0 to 200 nM, with a low detection limit of 2.48 and 3.01 nM for profenofos and isocarbophos, respectively. Moreover, it offers high selectivity and stability in real sample detection with high recoveries. Then, a self-designed portable smartphone sensing platform was successfully used for quantitative result outputs, demonstrating experience in designing a neotype sensing strategy for point-of-care pesticide monitoring. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1021/acs.analchem.4c01307Test; https://pubmed.ncbi.nlm.nih.gov/38808501Test |
| DOI: | 10.1021/acs.analchem.4c01307 |
| الإتاحة: | https://doi.org/10.1021/acs.analchem.4c01307Test https://pubmed.ncbi.nlm.nih.gov/38808501Test |
| رقم الانضمام: | edsbas.A45A7C7F |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.analchem.4c01307 |
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