دورية أكاديمية
EYA protein complex is required for Wntless retrograde trafficking from endosomes to Golgi.
| العنوان: | EYA protein complex is required for Wntless retrograde trafficking from endosomes to Golgi. |
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| المؤلفون: | Reshi, Hilal Ahmad, Medishetti, Raghavender, Ahuja, Aishwarya, Balasubramanian, Deepa, Babu, Kavita, Jaiswal, Manish, Chatti, Kiranam, Maddika, Subbareddy |
| المصدر: | Dev Cell ; ISSN:1878-1551 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2024 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | EYA protein, SCAMP3, TGN, Wnt signaling, Wntless, retromer, trans-Golgi network |
| الوصف: | Retrograde transport of WLS (Wntless) from endosomes to trans-Golgi network (TGN) is required for efficient Wnt secretion during development. However, the molecular players connecting endosomes to TGN during WLS trafficking are limited. Here, we identified a role for Eyes Absent (EYA) proteins during retrograde trafficking of WLS to TGN in human cell lines. By using worm, fly, and zebrafish models, we found that the EYA-secretory carrier-associated membrane protein 3 (SCAMP3) axis is evolved in vertebrates. EYAs form a complex and interact with retromer on early endosomes. Retromer-bound EYA complex recruits SCAMP3 to endosomes, which is necessary for the fusion of WLS-containing endosomes to TGN. Loss of EYA complex or SCAMP3 leads to defective transport of WLS to TGN and failed Wnt secretion. EYA mutations found in patients with hearing loss form a dysfunctional EYA-retromer complex that fails to activate Wnt signaling. These findings identify the EYA complex as a component of retrograde trafficking of WLS from the endosome to TGN. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.devcel.2024.05.021Test; https://pubmed.ncbi.nlm.nih.gov/38870942Test |
| DOI: | 10.1016/j.devcel.2024.05.021 |
| الإتاحة: | https://doi.org/10.1016/j.devcel.2024.05.021Test https://pubmed.ncbi.nlm.nih.gov/38870942Test |
| حقوق: | Copyright © 2024 Elsevier Inc. All rights reserved. |
| رقم الانضمام: | edsbas.27B1E55A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.devcel.2024.05.021 |
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