دورية أكاديمية
Divergent lactate dehydrogenase isoenzyme profile in cellular compartments of primate forebrain structures.
| العنوان: | Divergent lactate dehydrogenase isoenzyme profile in cellular compartments of primate forebrain structures. |
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| المؤلفون: | Duka, Tetyana, Collins, Zachary, Anderson, Sarah M, Raghanti, Mary Ann, Ely, John J, Hof, Patrick R, Wildman, Derek E, Goodman, Morris, Grossman, Lawrence I, Sherwood, Chet C |
| المصدر: | Mol Cell Neurosci ; ISSN:1095-9327 ; Volume:82 |
| بيانات النشر: | Elsevier Science |
| سنة النشر: | 2017 |
| المجموعة: | PubMed Central (PMC) |
| مصطلحات موضوعية: | Cellular subfractions, Haplorhine, Lactate dehydrogenase, Metabolism, Mitochondria, Primate forebrain, Strepsirrhine |
| الوصف: | The compartmentalization and association of lactate dehydrogenase (LDH) with specific cellular structures (e.g., synaptosomal, sarcoplasmic or mitochondrial) may play an important role in brain energy metabolism. Our previous research revealed that LDH in the synaptosomal fraction shifts toward the aerobic isoforms (LDH-B) among the large-brained haplorhine primates compared to strepsirrhines. Here, we further analyzed the subcellular localization of LDH in primate forebrain structures using quantitative Western blotting and ELISA. We show that, in cytosolic and mitochondrial subfractions, LDH-B expression level was relatively elevated and LDH-A declined in haplorhines compared to strepsirrhines. LDH-B expression in mitochondrial fractions of the neocortex was preferentially increased, showing a particularly significant rise in the ratio of LDH-B to LDH-A in chimpanzees and humans. We also found a significant correlation between the protein levels of LDH-B in mitochondrial fractions from haplorhine neocortex and the synaptosomal LDH-B that suggests LDH isoforms shift from a predominance of A-subunits toward B-subunits as part of a system that spatially buffers dynamic energy requirements of brain cells. Our results indicate that there is differential subcellular compartmentalization of LDH isoenzymes that evolved among different primate lineages to meet the energy requirements in neocortical and striatal cells. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1016/j.mcn.2017.04.007Test; https://pubmed.ncbi.nlm.nih.gov/28461219Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531073Test/ |
| DOI: | 10.1016/j.mcn.2017.04.007 |
| الإتاحة: | https://doi.org/10.1016/j.mcn.2017.04.007Test https://pubmed.ncbi.nlm.nih.gov/28461219Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5531073Test/ |
| حقوق: | Copyright © 2017 Elsevier Inc. All rights reserved. |
| رقم الانضمام: | edsbas.FFF5A946 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.mcn.2017.04.007 |
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