دورية أكاديمية
Comparison of avascular lymph node fragment transplantation techniques to optimize lymphangiogenesis in the minipig model
| العنوان: | Comparison of avascular lymph node fragment transplantation techniques to optimize lymphangiogenesis in the minipig model |
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| المؤلفون: | Hadamitzky, Catarina, Bruns, Frank, Gratz, Klaus-Friedrich, Schindewolffs, Lia, Roth, Katrin S., Werner, Martin, Gordon, Kristiana, Vogt, Peter M., Pabst, Reinhard |
| المصدر: | http://lobid.org/resources/99370671689906441Test#!, 45(1):55-64. |
| سنة النشر: | 2021 |
| المجموعة: | Publisso (ZB MED-Publikationsportal Lebenswissenschaften) |
| مصطلحات موضوعية: | Vascular endothelial growth factor-C, Lymph node transplantation, Surgical technique, Experimental Study, Lymphangiogenesis, Lymphoedema, Porcine animal model |
| الوصف: | Background!#!Secondary lymphoedema is a challenging pandemic. This condition may arise after oncologic resection of tumor-draining lymph nodes and/or radiation. Plastic-surgical procedures for lymphoedema comprise transplantation of vascularized lymph node flaps, which are, however, technically challenging and difficult to implement on a global level due to the scarcity of microsurgery facilities in some countries. To improve this situation, comparative research in valid animal models is needed.!##!Methods!#!A total of 33 minipigs were subjected to lymphatic resection in the hind limbs. This large animal model was used in a first phase to compare different lymph node fragmentation methods and assess lymphatic regeneration after avascular transplantation. In a second phase, several stimulants were tested for their effect on lymphatic regeneration after fragment transplantation. In a third phase, animals additionally received irradiation of the groin. In this novel animal model, autologous avascular lymph node fragment transplantation was complemented by peripheral injections of vascular endothelial growth factor-C (VEGF-C). Finally, regeneration rates were quantified in relative numbers (percentage) in the irradiated tissue.!##!Results!#!In the first phase, transversal lymph node fragmentation under preservation of the nodal capsule showed the best percentage of regeneration (62.5%). Peripheral intradermal administration of VEGF-C enhanced lymph node fragment regeneration (70.8%) better than injections of tetanus toxoid (41.6%) or !##!Conclusions!#!The present findings provide a pre-clinical proof-of-concept for a possible simplification strategy for current operative procedures of autologous lymph node transplantation.Level of evidence : Not gradable. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | https://repository.publisso.de/resource/frl:6447927Test; https://doi.org/10.1007/s00238-021-01869-3Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818007Test/ |
| DOI: | 10.1007/s00238-021-01869-3 |
| الإتاحة: | https://doi.org/10.1007/s00238-021-01869-3Test https://repository.publisso.de/resource/frl:6447927Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8818007Test/ |
| حقوق: | https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.7015B736 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00238-021-01869-3 |
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