دورية أكاديمية
Impact of T-cell-mediated immune response on xenogeneic heart valve transplantation: Short-term success and mid-term failure
| العنوان: | Impact of T-cell-mediated immune response on xenogeneic heart valve transplantation: Short-term success and mid-term failure |
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| المؤلفون: | Biermann, Anna, Marzi, Julia, Brauchle, Eva, Schneider, Maria, Kornberger, Angela, Abdelaziz, Sherif, Wichmann, Julian L., Arendt, Chirstophe T., Nagel, Eike, Brockbank, Kelvin, Seifert, Martina, Schenke-Layland, Katja, Stock, Ulrich A. |
| سنة النشر: | 2018 |
| المجموعة: | Publikationsdatenbank der Fraunhofer-Gesellschaft |
| مصطلحات موضوعية: | ice-free cryopreservation, heart valve transplantation, Allograft, Xenograft, T-cell-mediated immune response, organ scarcity |
| الوقت: | 660, 616, 610, 620 |
| الوصف: | S.784-792 ; OBJECTIVES: Allogeneic frozen cryopreserved heart valves (allografts or homografts) are commonly used in clinical practice. A major obstacle for their application is the limited availability in particular for paediatrics. Allogeneic large animal studies revealed that alternative ice-free cryopreservation (IFC) results in better matrix preservation and reduced immunogenicity. The objective of this study was to evaluate xenogeneic (porcine) compared with allogeneic (ovine) IFC heart valves in a large animal study. METHODS: IFC xenografts and allografts were transplanted in 12 juvenile merino sheep for 1-12 weeks. Immunohistochemistry, ex vivo computed tomography scans and transforming growth Factor-V release profiles were analysed to evaluate postimplantation immunopathology. In addition, near-infrared multiphoton imaging and Raman spectroscopy were employed to evaluate matrix integrity of the leaflets. RESULTS: Acellular leaflets were observed in both groups 1 week after implantation. Allogeneic leaflets remained acellular throughout the entire study. In contrast, xenogeneic valves were infiltrated with abundant T-cells and severely thickened over time. No collagen or elastin changes could be detected in either group using multiphoton imaging. Raman spectroscopy with principal component analysis focusing on matrix-specific peaks confirmed no significant differences for explanted allografts. However, xenografts demonstrated clear matrix changes, enabling detection of distinct inflammatory-driven changes but without variations in the level of transforming growth factor-v. CONCLUSIONS: Despite short-term success, mid-term failure of xenogeneic IFC grafts due to a T-cell-mediated extracellular matrixtriggered immune response was shown. ; 53 ; Nr.4 |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | European journal of cardio-thoracic surgery; https://publica.fraunhofer.de/handle/publica/254495Test |
| DOI: | 10.1093/ejcts/ezx396 |
| الإتاحة: | https://doi.org/10.1093/ejcts/ezx396Test https://publica.fraunhofer.de/handle/publica/254495Test |
| رقم الانضمام: | edsbas.CA570E42 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1093/ejcts/ezx396 |
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