دورية أكاديمية
Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don
| العنوان: | Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don |
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| المؤلفون: | Massonneau, A, Martinoia, E, Dietz, Karl-Josef, Mimura, T |
| بيانات النشر: | SPRINGER-VERLAG |
| سنة النشر: | 2000 |
| المجموعة: | PUB - Publications at Bielefeld University |
| مصطلحات موضوعية: | vacuole, tonoplast, acid phosphate transport, 2 '-disulfonic, chromate, 4-diisothio cyanostilbene-2, Catharanthus |
| الوقت: | 4 |
| الوصف: | Massonneau A, Martinoia E, Dietz K-J, Mimura T. Phosphate uptake across the tonoplast of intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don. PLANTA . 2000;211(3):390-395. ; Transport of inorganic orthophosphate (Pi) across the tonoplast membrane was studied using intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus. Orthophosphate uptake was strongly stimulated in the presence of Mg-ATP and Mg-pyrophosphate and inhibited by bafilomycin and concanamycin which are potent inhibitors of the vacuolar H+-ATPase. These results indicated that the buildup of an electrochemical gradient by the H+ pumps tvas essential for the uptake of Pi. Potassium thiocyanate, which dissipates the membrane potential across the tonoplast, strongly inhibited the Mg-ATP-stimulated uptake of Pi, while only a weak inhibition was observed in the presence of NH4Cl, which dissipates the pH gl-adient. These results indicate that, as observed for other anions like malate or chloride, the electrical component is the driving force of Pi uptake, whereas the Delta pH plays only a minor role. Possible competitive inhibitors of Pi, MoO42-, VO43- and CrO42- were tested. Among them, CrO42- strongly inhibited Pi uptake into the vacuoles. Various inhibitors of anion transport were also tested. Only 4,4-diisothiocyanostilbene-2,2'-disulfonic acid strongly inhibited Pi uptake into the vacuoles. The function of the vacuolar Pi transporters for cytoplasmic Pi homeostasis is discussed. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/issn/0032-0935; info:eu-repo/semantics/altIdentifier/issn/1432-2048; info:eu-repo/semantics/altIdentifier/wos/000088843600012; info:eu-repo/semantics/altIdentifier/pmid/10987558; https://pub.uni-bielefeld.de/record/1619196Test |
| الإتاحة: | https://doi.org/10.1007/s004250000297Test https://pub.uni-bielefeld.de/record/1619196Test |
| حقوق: | info:eu-repo/semantics/closedAccess |
| رقم الانضمام: | edsbas.F9F7C2E7 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |