دورية أكاديمية
A comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS.
| العنوان: | A comprehensive spectral assay library to quantify the Halobacterium salinarum NRC-1 proteome by DIA/SWATH-MS. |
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| المؤلفون: | Kusebauch, Ulrike, Lorenzetti, Alan P R, Campbell, David S, Pan, Min, Shteynberg, David, Kapil, Charu, Midha, Mukul K, López García de Lomana, Adrián, Baliga, Nitin, Moritz, Robert L |
| المصدر: | Articles, Abstracts, and Reports |
| بيانات النشر: | Providence Digital Commons |
| سنة النشر: | 2023 |
| المجموعة: | Providence St. Joseph Health Digital Commons |
| مصطلحات موضوعية: | washington, isb, Halobacterium salinarum, Peptides, Proteome, Proteomics |
| الوصف: | Data-Independent Acquisition (DIA) is a mass spectrometry-based method to reliably identify and reproducibly quantify large fractions of a target proteome. The peptide-centric data analysis strategy employed in DIA requires a priori generated spectral assay libraries. Such assay libraries allow to extract quantitative data in a targeted approach and have been generated for human, mouse, zebrafish, E. coli and few other organisms. However, a spectral assay library for the extreme halophilic archaeon Halobacterium salinarum NRC-1, a model organism that contributed to several notable discoveries, is not publicly available yet. Here, we report a comprehensive spectral assay library to measure 2,563 of 2,646 annotated H. salinarum NRC-1 proteins. We demonstrate the utility of this library by measuring global protein abundances over time under standard growth conditions. The H. salinarum NRC-1 library includes 21,074 distinct peptides representing 97% of the predicted proteome and provides a new, valuable resource to confidently measure and quantify any protein of this archaeon. Data and spectral assay libraries are available via ProteomeXchange (PXD042770, PXD042774) and SWATHAtlas (SAL00312-SAL00319). |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.providence.org/publications/7935Test; https://pubmed.ncbi.nlm.nih.gov/37833331Test |
| الإتاحة: | https://digitalcommons.providence.org/publications/7935Test https://pubmed.ncbi.nlm.nih.gov/37833331Test |
| رقم الانضمام: | edsbas.DC3DC633 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |