دورية أكاديمية
Determination of Vitamin C in Small Volumes of Blood by HPLC/EC: Determination of vitamin C in blood
| العنوان: | Determination of Vitamin C in Small Volumes of Blood by HPLC/EC: Determination of vitamin C in blood |
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| المؤلفون: | G. Watson, David, Lotfollahzadeh, Samad, Mokhber Dezfoulib, Mohammad Reza, Hadjiakhoondic, Abbas |
| المصدر: | Iranian Journal of Pharmaceutical Sciences; Vol. 1 No. 1 (2005): IJPS_Volume 1_Issue 1; 7-10 ; 1735-2444 |
| بيانات النشر: | Iranian Journal of Pharmaceutical Sciences |
| سنة النشر: | 2005 |
| المجموعة: | Portal of Scientific Journals at Shahid Beheshti University of Medical Science |
| مصطلحات موضوعية: | Vitamin C, Electrochemical detection, HPLC, Blood |
| الوصف: | A sensitive procedure for determining total vitamin C (ascorbic acid + dehydrate ascorbic Acid) in a blood drop from a finger prick, before and after the administration of a vitamin C tablet is described. Analysis was carried out by high performance liquid chromatography with electrochemical detection (HPLC/EC).Measurements were taken one hour, two hours and six hours after the administration of a 500 mg vitamin C tablet. D-Isoascorbic acid was used as the internalstandard and analysis was carried out using two C-18 columns connected in series and a phosphate buffer mobile phase. Dehydroascorbic acid in the samples was converted to ascorbic acid by incubation with DL-homocysteine for 30 minutes. The level of vitamin C in blood reached a maximum concentration after twohours. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | http://journals.sbmu.ac.ir/index.php/ijps/article/view/39436/29408Test; http://journals.sbmu.ac.ir/index.php/ijps/article/view/39436Test |
| DOI: | 10.22037/ijps.v1.39436 |
| الإتاحة: | https://doi.org/10.22037/ijps.v1.39436Test http://journals.sbmu.ac.ir/index.php/ijps/article/view/39436Test |
| رقم الانضمام: | edsbas.17090419 |
| قاعدة البيانات: | BASE |
| DOI: | 10.22037/ijps.v1.39436 |
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