دورية أكاديمية
Arsenic trioxide promotes mitochondrial DNA mutation and cell apoptosis in primary APL cells and NB4 cell line
| العنوان: | Arsenic trioxide promotes mitochondrial DNA mutation and cell apoptosis in primary APL cells and NB4 cell line |
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| المؤلفون: | Meng Ran, Zhou Jin, Sui Meng, Li ZhiYong, Feng GuoSheng, Yang BaoFeng |
| المساهمون: | Meng, R (reprint author), Beijing Univ, Shijitan Hosp, Div Neurol, Clin Med Coll 9, Beijing 100038, Peoples R China., Beijing Univ, Shijitan Hosp, Div Neurol, Clin Med Coll 9, Beijing 100038, Peoples R China., Harbin Med Coll, Hosp 1, Harbin 150001, Peoples R China., Univ Int Business & Econ, Beijing 100029, Peoples R China., Addenbrookes Hosp Cambridge, Cambridge Univ Hosp NHS Fdn Trust, Dept Radiol, Cambridge CB2 0QQ, England. |
| المصدر: | SCI |
| بيانات النشر: | science china life sciences |
| سنة النشر: | 2010 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | arsenical, mitochondrial gene, mutation, D-LOOP REGION, GENE-EXPRESSION, LEUKEMIC-CELLS, BLADDER-CANCER, MYELOMA CELLS, PROFILES, KINASE, TARGET, GROWTH |
| الوصف: | This study aimed to investigate the effects of arsenic trioxide (As(2)O(3)) on the mitochondrial DNA (mtDNA) of acute promyelocytic leukemia (APL) cells. The NB4 cell line was treated with 2.0 mu mol/L As(2)O(3) in vitro, and the primary APL cells were treated with 2.0 mu mol/L As(2)O(3) in vitro and 0.16 mg kg(-1) d(-1) As(2)O(3) in vivo. The mitochondrial DNA of all the cells above was amplified by PCR, directly sequenced and analyzed by Sequence Navigatore and Factura software. The apoptosis rates were assayed by flow cytometry. Mitochondrial DNA mutation in the D-loop region was found in NB4 and APL cells before As(2)O(3) use, but the mutation spots were remarkably increased after As(2)O(3) treatment, which was positively correlated to the rates of cellular apoptosis, the correlation coefficient: r(NB4-As2O3)=0.973818, and r(APL-As2O3)=0.934703. The mutation types include transition, transversion, codon insertion or deletion, and the mutation spots in all samples were not constant and regular. It is revealed that As(2)O(3) aggravates mtDNA mutation in the D-loop region of acute promyelocytic leukemia cells both in vitro and in vivo. Mitochondrial DNA might be one of the targets of As(2)O(3) in APL treatment. ; Biology ; SCI(E) ; 11 ; ARTICLE ; 1 ; 87-93 ; 53 |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | English |
| ردمك: | 978-0-00-276437-7 0-00-276437-7 |
| تدمد: | 1674-7305 |
| العلاقة: | SCIENCE CHINA-LIFE SCIENCES.2010,53,(1),87-93.; 1021226; http://hdl.handle.net/20.500.11897/401315Test; WOS:000276437700011 |
| DOI: | 10.1007/s11427-010-0004-9 |
| الإتاحة: | https://doi.org/20.500.11897/401315Test https://doi.org/10.1007/s11427-010-0004-9Test https://hdl.handle.net/20.500.11897/401315Test |
| رقم الانضمام: | edsbas.3FFA5C19 |
| قاعدة البيانات: | BASE |
| ردمك: | 9780002764377 0002764377 |
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| تدمد: | 16747305 |
| DOI: | 10.1007/s11427-010-0004-9 |