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A Point Mutation in DNA Polymerase beta (POLB) Gene Is Associated with Increased Progesterone Receptor (PR) Expression and Intraperitoneal Metastasis in Gastric Cancer

التفاصيل البيبلوغرافية
العنوان: A Point Mutation in DNA Polymerase beta (POLB) Gene Is Associated with Increased Progesterone Receptor (PR) Expression and Intraperitoneal Metastasis in Gastric Cancer
المؤلفون: Tan, Xiaohui, Wu, Xiaoling, Ren, Shuyang, Wang, Hongyi, Li, Zhongwu, Alshenawy, Weaam, Li, Wenmei, Cui, Jiantao, Luo, Guangbin, Siegel, Robert S., Fu, Sidney W., Lu, Youyong
المساهمون: Lu, YY (reprint author), Peking Univ, Key Lab Carcinogenesis & Translat Res, Beijing Canc Hosp & Inst, Lab Mol Oncol,Minist Educ,Sch Oncol, 52 Fu Cheng Lu, Beijing 100142, Peoples R China., Fu, SW (reprint author), George Washington Univ, Sch Med & Hlth Sci, Dept Med, 2300 Eye St NW,Ross Hall 402C, Washington, DC 20037 USA., Peking Univ, Key Lab Carcinogenesis & Translat Res, Beijing Canc Hosp & Inst, Lab Mol Oncol,Minist Educ,Sch Oncol, 52 Fu Cheng Lu, Beijing 100142, Peoples R China., Chengdu Mil Gen Hosp, Dept Gastroenterol, Chengdu, Peoples R China., Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Sugary, 52 Fu Cheng Lu, Beijing 100142, Peoples R China., Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Dept Pathol, 52 Fu Cheng Lu, Beijing 100142, Peoples R China., Case Western Reserve Univ, Dept Genet, Cleveland, OH 44106 USA., George Washington Univ, Sch Med & Hlth Sci, Dept Med, 2300 Eye St NW,Ross Hall 402C, Washington, DC 20037 USA.
المصدر: SCI ; PubMed
بيانات النشر: JOURNAL OF CANCER
سنة النشر: 2016
المجموعة: Peking University Institutional Repository (PKU IR) / 北京大学机构知识库
مصطلحات موضوعية: Gastric cancer, DNA polymerase beta, progesterone receptor, metastasis, BASE EXCISION-REPAIR, BREAST-CANCER, CELLULAR-TRANSFORMATION, TRANSLOCATION, DEFICIENCY, ESTROGEN, BRCA1, CELLS
الوصف: Increased expression of progesterone receptor (PR) has been reported in gastric cancer (GC). We have previously identified a functional T889C point mutation in DNA polymerase beta (POLB), a DNA repair gene in GC. To provide a detailed analysis of molecular changes associated with the mutation, human cDNA microarrays focusing on 18 signal transduction pathways were used to analyze differential gene expression profiles between GC tissues with T889C mutant in POLB gene and those with wild type. Among the differentially expressed genes, notably, PR was one of the significantly up-regulated genes in T889C mutant POLB tissues, which were subsequently confirmed in POLB gene transfected AGS cell line. Interestingly, patients with T889C mutation and PR positivity were associated with higher incidence of intraperitoneal metastasis (IM). In vitro studies indicate that PR expression was upregulated in AGS cell line when transfected with T889C mutant expression vector. Cotransfection of T889C mutant allele and PR gene induced cell migration in the cell line. These data demonstrated that T889C mutation-associated PR overexpression results in increased IM. Therefore, T889C mutation-associated PR overexpression may serve as a biomarker for an adverse prognosis for human GC. ; National Key Basic Research Program Project of China [2004CB518708]; National Natural Science Foundation of China (NSFC) [39625016]; National Bio-Tech 863 Program [2012-AA02A203]; Elaine H. Snyder Cancer Research Award ; SCI(E) ; PubMed ; ARTICLE ; sfu@gwu.edu; youyonglu@sina.com ; 11 ; 1472-1480 ; 7
نوع الوثيقة: journal/newspaper
اللغة: English
تدمد: 1837-9664
العلاقة: JOURNAL OF CANCER.2016,7(11),1472-1480.; 1436752; http://hdl.handle.net/20.500.11897/492500Test; WOS:000380050300012
DOI: 10.7150/jca.14844
الإتاحة: https://doi.org/20.500.11897/492500Test
https://doi.org/10.7150/jca.14844Test
https://hdl.handle.net/20.500.11897/492500Test
رقم الانضمام: edsbas.4DA328FA
قاعدة البيانات: BASE
الوصف
تدمد:18379664
DOI:10.7150/jca.14844