دورية أكاديمية
外阴阴道念珠菌病白念珠菌交配型基因分析及其致病性研究 ; Mating-Type Locus and virulence in Candida albicans from vulvovaginal candidiasis
| العنوان: | 外阴阴道念珠菌病白念珠菌交配型基因分析及其致病性研究 ; Mating-Type Locus and virulence in Candida albicans from vulvovaginal candidiasis |
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| المؤلفون: | 单莹莹, 李健玲, 黎婷, 刘小平, 樊尚荣 |
| المساهمون: | 北京大学深圳医院 妇产科,深圳,518036, 北京大学深圳医院 检验科,深圳,518036, 北京大学深圳医院 妇产科，深圳 518036, 深圳市科创委妇科疾病重点实验室，深圳 518036 |
| المصدر: | CSCD ; 知网 ; 万方 ; http://d.g.wanfangdata.com.cn/Periodical_xdfckjz201507001.aspxTest |
| بيانات النشر: | 现代妇产科进展 |
| سنة النشر: | 2015 |
| المجموعة: | Peking University Institutional Repository (PKU IR) / 北京大学机构知识库 |
| مصطلحات موضوعية: | 外阴阴道念珠菌病, 白念珠菌, 交配型基因, ASL3, HWP1, ASP1, Vulvovaginal candidiasis, Candida albicans, Mating type |
| الوصف: | 目的：：研究临床致病白念珠菌交配型基因MTL纯合子与杂合子的流行病学特点,分析其与外阴阴道念珠菌病患者的临床特征、白念珠菌致病力及毒力的关系。方法：通过普通PCR对临床外阴阴道念珠菌病患者阴道分泌物分离的致病白念珠菌MTL基因进行检测,应用实时荧光定量PCR反应分析MTL纯合子与杂合子中毒力相关基因ASL3、SAP1和HWP1基因的表达,并采用Rosco药片扩散法对白念珠菌进行体外药物敏感试验。结果：1338株白念珠菌中,1330株为MTLa/α杂合子,8株为MTL纯合子(7株MTLa/a纯合子,1株MTLα/α纯合子)。8株MTL纯合子所致外阴阴道念珠菌病患者中6例患者达到真菌学治愈。与杂合子相比,白念珠菌MTL纯合子对唑类药物耐药性较高(0~75％ vs 0~2.7％,P<0.05)。毒力相关基因ASL3在两者中的表达无明显统计学差异,SAP1在MTL杂合子中表达稍高(1.02±0.68 vs 0.83±0.31,P>0.05),而HWP1在杂合子中表达明显增高(1.71±1.42 vs 1.20±0.62,P<0.05)。结论：外阴阴道念珠菌病白念珠菌MTL纯合子在外阴阴道念珠菌病患者中流行率较低；与杂合子相比, MTL纯合子耐药性增加；而毒力相关基因HWP1、SAP1和ASL3在纯合子中表达降低,这与其临床特征相符。 ; Objective:To analyse the epidemiological characteristics of MTL locus het-erozygous isolates and MTL locus homozygous isolates,and the relationship between the MTL lo-cus and clinical characteristics of vulvovaginal candidiasis ( VVC ) patients, pathogenicity or virulence of Candida albicans. Methods:The PCR was used to detect the MTL genes in Candi-da albicans,which were isolated from vaginal secretions of VVC patients. Real-time fluorescent quantitative PCR reaction was applicated to analyze the expression of virulence factors HWP1、SAP1 and ASL3 . Drug sensitivity tests in vitro were also performed by Rosco tablets diffusion method. Result:We detected 1338 Candida albicans isolates and found 1330 MTL locus hetero-zygous isolates and 8 were MTL locus homozygous isolates,of which 7 isolates were MTLa/a and one isolate was MTLα/α. Six of eight patients with VVC caused by C albicans MTL locus homo-zygous isolates were cured. The azoles resistant rates of homozygous isolates were higher than those of heterozygous isolates (0~75% vs 0~2. 7%,P<0. 05). Expression levels of virulence-related genes ASL3 were similar in heterozygous isolates and in homozygous isolates ( 0 . 35 ±0. 32 vs 0. 27±0. 25,P>0. 05);SAP1 were a little higher in heterozygous isolates than in homo-zygous isolates (1. 02±0. 68 vs 0. 83±0. 31,P>0. 05);HWP1 were significantly higher in het-erozygous isolates than in homozygous isolates (1. 71±1. 42 vs 1. 20±0. 62,P<0. ... |
| نوع الوثيقة: | journal/newspaper |
| اللغة: | Chinese |
| تدمد: | 1004-7379 |
| العلاقة: | 现代妇产科进展.2015,(7),481-486.; 1286223; http://hdl.handle.net/20.500.11897/414327Test |
| DOI: | 10.13283/j.cnki.xdfckjz.2015.07.001 |
| الإتاحة: | https://doi.org/20.500.11897/414327Test https://doi.org/10.13283/j.cnki.xdfckjz.2015.07.001Test https://hdl.handle.net/20.500.11897/414327Test |
| رقم الانضمام: | edsbas.F067B774 |
| قاعدة البيانات: | BASE |
| تدمد: | 10047379 |
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| DOI: | 10.13283/j.cnki.xdfckjz.2015.07.001 |