دورية أكاديمية
Expression of Toll-Like Receptors in the Lung Tissue of Mouse Fetuses Generated by in vitro Embryo Culture and Embryo Transfer
| العنوان: | Expression of Toll-Like Receptors in the Lung Tissue of Mouse Fetuses Generated by in vitro Embryo Culture and Embryo Transfer |
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| المؤلفون: | DoÄŸan, Göksel, Karagenç, Nedim, Esmen, Kerem, Çınar Kul, Bengi, YeÅŸilkaya, Hasan, Akgün, Åžakir, Orman, Mehmet Nurullah, Sandikci, Mustafa, Eren, Ulker, Unsal, Humeyra, Karagenc, Levent |
| بيانات النشر: | Karger |
| سنة النشر: | 2023 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | Assisted reproduction, In vitro embryo culture, Lung development, Toll-like receptors, Toll-like receptor signaling pathway, Lipopolysaccharide-Binding Protein, Assisted Reproductive Technology, Low-Birth-Weight, Perinatal Outcomes, Epithelial-Cells, Developmental Expression, Singleton Pregnancies, Negative Regulator, Immune-Responses, Dendritic Cells |
| الوصف: | Article; Early Access ; Mouse fetuses generated by in vitro embryo culture and embryo transfer exhibit impaired lung development, altered composition of pulmonary epithelial cells associated with downregulation of several genes involved in lung development and toll-like receptor (TLR) signaling pathway. The aims of the present study were to determine the expression of all TLRs and to examine if the expression of TLRs, along with genes involved in TLR signaling pathway, is altered in the lung tissue of mouse fetuses generated through embryo culture and embryo transfer. Two experimental (EGs) and one control (CG) group were included in the study. Embryos cultured at 5% CO2-95% air for 95 h or less than 24 h were transferred to pseudo-pregnant females to obtain fetuses comprising EG(in vitro) (n = 18) and EG(in vivo) (n = 18), respectively. Fetuses obtained from naturally ovulating females on day 18 of pregnancy served as the CG (n = 18). Western blot and immunohistochemistry were used to determine the expression of TLR proteins. The expression of transcripts encoding TLRs, and the genes involved in TLR signaling pathway (Lbp, Pik3r1, Pik3cb, Nfkbia, and Fos), was determined using qRT-PCR. While all TLRs were expressed by cells lining the bronchial/bronchiolar epithelium of lung tissues in all groups, some of the TLRs were expressed in a specific pattern. When compared to CG, the expression of transcripts encoding TLR-2, -3, -4, -5, -7, -8, -9, -12, -13, Lbp, Pik3r1, Pik3cb, Nfkbia, and Fos was significantly downregulated in both EGs. It appears that stress imposed on embryos at preimplantation stages of development is associated with downregulation of TLRs, along with some of the genes involved in TLR signaling pathway, in the lung tissue during the perinatal period. It remains to be determined if downregulation of TLRs, along with the genes involved in TLR signaling pathway, has any functional consequences in the adult lung tissue. ; Scientific and Technological Research Council of Turkey (TUBITAK) [112O259] ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1422-6405 1422-6421 |
| العلاقة: | Cells Tissues Organs; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/51983Test; https://doi.org/10.1159/000529974Test; WOS:001010222800001 |
| DOI: | 10.1159/000529974 |
| الإتاحة: | https://doi.org/10.1159/000529974Test https://hdl.handle.net/11499/51983Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.F8574808 |
| قاعدة البيانات: | BASE |
| تدمد: | 14226405 14226421 |
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| DOI: | 10.1159/000529974 |