دورية أكاديمية
Role of AHR, NF-kB and CYP1A1 crosstalk with the X protein of Hepatitis B virus in hepatocellular carcinoma cells
| العنوان: | Role of AHR, NF-kB and CYP1A1 crosstalk with the X protein of Hepatitis B virus in hepatocellular carcinoma cells |
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| المؤلفون: | Olmez N., Koc T., Semiz A., Lale Satiroglu-Tufan N., Celik-Turgut G., Dodurga Y., Sen, Alaattin, Ozgun-Acar O. |
| بيانات النشر: | Elsevier B.V. |
| سنة النشر: | 2023 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | Aryl hydrocarbon receptor, Cytochrome P4501A1, Hepatitis B virus X protein, Hepatocellular carcinoma, Nuclear factor-kappa B, aromatic hydrocarbon receptor, cytochrome P450 1A1, firefly luciferase, immunoglobulin enhancer binding protein, Renilla luciferin 2 monooxygenase, AHR gene, Article, carcinogenicity, cell viability, coimmunoprecipitation, controlled study, CYP1A1 gene, gene expression, gene interaction, Hep-G2 cell line, human, human cell, inflammation, liver cell carcinoma, luciferase assay, molecular interaction, NF kB gene, NF kB signaling, protein expression, protein protein interaction |
| الوصف: | In this study, it was aimed to elucidate the interaction between aryl hydrocarbon receptor (AHR), nuclear factor-kappa B (NF-kB), and cytochrome P4501A1 (CYP1A1) with hepatitis B virus X protein (HBX) in a human liver cancer cell line (HepG2) transfected with HBX. First, AHR, NF-kB, and CYP1A1 genes were cloned into the appropriate region of the CheckMate mammalian two-hybrid recipient plasmids using a flexi vector system. Renilla and firefly luciferases were quantified using the dual-luciferase reporter assay system to measure the interactions. Secondly, transient transfections of CYP1A1 and NF-kB (RelA) were performed into HBX–positive and HBX–negative HepG2 cells. The mRNA expression of CYP1A1 and NF-kB genes were confirmed with RT-PCR, and cell viability was measured by WST-1. Further verification was assessed by measuring the activity and protein level of CYP1A1. Additionally, CYP1A1/HBX protein–protein interactions were performed with co-immunoprecipitation, which demonstrated no interaction. These results have clearly shown that the NF-kB and AHR genes interact with HBX without involving CYP1A1 and HBX protein–protein interactions. The present study confirms that AHR and NF-kB interaction plays a role in the HBV mechanism mediated via HBX and coordinating the carcinogenic or inflammatory responses; still, the CYP1A1 gene has no effect on this interaction. © 2022 Elsevier B.V. ; 2011FBE053, 2014FBE028; Türkiye Bilimsel ve Teknolojik AraÅŸtırma Kurumu, TÃœBÄ°TAK: 111T612 ; This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) [Grant No 111T612 ]; and Pamukkale University Department of Scientific Research Projects (PAU-BAP) [grant number 2011FBE053 and 2014FBE028]. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0378-1119 |
| العلاقة: | Gene; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://doi.org/10.1016/j.gene.2022.147099Test; https://hdl.handle.net/11499/47438Test; 853; 2-s2.0-85143722253; WOS:000904587300001 |
| DOI: | 10.1016/j.gene.2022.147099 |
| الإتاحة: | https://doi.org/10.1016/j.gene.2022.147099Test https://hdl.handle.net/11499/47438Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.9CA2AD74 |
| قاعدة البيانات: | BASE |
| تدمد: | 03781119 |
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| DOI: | 10.1016/j.gene.2022.147099 |