دورية أكاديمية
The effect of in ovo ethanol exposure on retina and optic nerve in a chick embryo model system
| العنوان: | The effect of in ovo ethanol exposure on retina and optic nerve in a chick embryo model system |
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| المؤلفون: | Tufan, Ahmet Çevik., Abban, Mete Gülçin, Akdogan, I., Erdogan, D., Ozogul, C. |
| سنة النشر: | 2007 |
| المجموعة: | Pamukkale University Repository / Pamukkale Üniversitesi Açık Erişim Arşivi |
| مصطلحات موضوعية: | Chick embryo, Fetal alcohol syndrome, Optic nerve, Retina, Retinal pigment epithelium, alcohol, melanin, myelin, animal tissue, article, cell death, controlled study, disease association, electron microscopy, embryo, embryo growth, eye malformation, fertilization, humidity, intrauterine growth retardation, microscopy, myelination, nonhuman, pigment epithelium, pigmentation, prenatal exposure, retina development, retina ganglion cell, ultrastructure, Abnormalities |
| الوصف: | Ocular anomalies seen in children with fetal alcohol syndrome (FAS) suggest that ocular structures are sensitive to alcohol exposure during their development. This study was designed to investigate the effect of in ovo ethanol (EtOH) exposure on retinal development and myelinization of optic nerve fibers at an ultra structural level in a chick embryo model system. Prior to incubation, fertilized chicken eggs were injected once with 100 µl of either 0.9% NaCl (vehicle control), or EtOH solutions at different doses (10, 30, or 50%, v:v in 0.9% NaCl) into their air sacs and incubated at 37.5 °C and saturation humidity. On day 20 embryos were analyzed in terms of their viability and growth and the optic cups including the optic nerves were dissected out. Specimens were processed for electron microscopy (EM). Results showed that, EtOH significantly decreased the viability of chick embryos (P 0.045), and caused significant prenatal growth retardation (P 0.004) in a dose-dependant manner. Light microscopy of semi thin sections revealed that prenatal exposure to EtOH resulted in both retinal degeneration and optic nerve hypoplasia (P 0.001) in a dose-dependant manner. EM revealed that a dose-dependant decrease in the number of myelinated nerve fibers was profound in groups exposed to EtOH (P 0.001). Furthermore, the myelin coats observed were thinner than those seen in control embryos. In groups exposed to EtOH myelin sheets were unorganized and contained vacuolar structures in between them. The tissue in between the cells and optic nerve fibers, on the other hand, lost its intact appearance with vacuolar and vesicular structures in between them. In addition, the optic nerve fibers contained granular accumulations in EtOH exposed groups. A dose dependent degeneration was also observed in retinas of EtOH exposed groups. The effect of EtOH was profound in pigment epithelium (PE), inner plexiform layer (IPL), and ganglion cell layer (GC). Mitochondrial deficiencies, and alterations in melanin granule number and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0890-6238 |
| العلاقة: | Reproductive Toxicology; Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı; https://hdl.handle.net/11499/4397Test; https://doi.org/10.1016/j.reprotox.2006.09.006Test; 23; 75; 82; 2-s2.0-33845866865; WOS:000243772800008 |
| DOI: | 10.1016/j.reprotox.2006.09.006 |
| الإتاحة: | https://doi.org/10.1016/j.reprotox.2006.09.006Test https://hdl.handle.net/11499/4397Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.110BBF52 |
| قاعدة البيانات: | BASE |
| تدمد: | 08906238 |
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| DOI: | 10.1016/j.reprotox.2006.09.006 |