التفاصيل البيبلوغرافية
| العنوان: |
Studies on grape, wine and grape seed proteins and development of methods for their biochemical and functional characterization |
| المؤلفون: |
Gazzola, Diana |
| المساهمون: |
Gazzola, Diana, Curioni, Andrea, Corich, Viviana |
| بيانات النشر: |
Università degli studi di Padova |
| سنة النشر: |
2012 |
| المجموعة: |
Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: |
aggregates, chitinase, grape seed storage proteins, protein quantification, protein haze, thaumatin-like proteins, wine proteins, Settore AGR/15 - Scienze e Tecnologie Alimentari |
| الوصف: |
Grape and wine proteins represent a subject of study that in recent years has received increasing attention from the international research, mainly due to the fact that important issues on wine can be clarified through the study of the nature and the properties of these macromolecules. Among the reasons of increased concern for winemakers there is the problem of haze development in bottled white wines, known as “protein casse”, due to the presence of residual amounts of insoluble proteins that can become unstable and precipitate during wine storage, causing the appearance of sediments and turbidity. These precipitates are generally the result of denaturation and subsequent aggregation of heat-unstable wine proteins deriving from grapes and belonging to the functional category of plant pathogenesis-related (PR) proteins, namely thaumatin-like proteins (TLPs) and chitinases. It has been observed that these proteins are resistant to acidic pH, proteolysis and fermentation conditions, hence they survive the winemaking process. In this thesis, firstly, the knowledge about chitinases was elaborated since recent scientific papers have described them as proteins potentially more susceptible to precipitation in white wines. In this context, several chitinase isoforms have been purified from Manzoni Bianco grape juice and their electrophoretic behaviour was characterized, deducing important functional and biochemical information on the properties of these enzymes. Subsequently, the research has focused on white wine protein aggregation by means of an innovative instrument, the Izon qNano, for polydisperse nanoparticles detection and quantitation in heat-tested samples. In detail, the role towards aggregates formation upon heating played by TLPs, chitinase, phenolics and polysaccharides, all purified from the same unfined white wine, was investigated via reconstitution experiments to better understand the contribution of each compound on haze formation. Taking into account both the high number and the big size of ... |
| نوع الوثيقة: |
doctoral or postdoctoral thesis |
| اللغة: |
English |
| العلاقة: |
numberofpages:187; http://hdl.handle.net/11577/3422114Test |
| الإتاحة: |
http://hdl.handle.net/11577/3422114Test |
| حقوق: |
info:eu-repo/semantics/openAccess |
| رقم الانضمام: |
edsbas.57FFFF2D |
| قاعدة البيانات: |
BASE |
