دورية أكاديمية
Optimization of 1,2,4-Triazole-3-thiones toward Broad-Spectrum Metallo-β-lactamase Inhibitors Showing Potent Synergistic Activity on VIM- and NDM-1-Producing Clinical Isolates
| العنوان: | Optimization of 1,2,4-Triazole-3-thiones toward Broad-Spectrum Metallo-β-lactamase Inhibitors Showing Potent Synergistic Activity on VIM- and NDM-1-Producing Clinical Isolates |
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| المؤلفون: | Legru, Alice, Verdirosa, Federica, Vo-Hoang, Yen, Tassone, Giusy, Vascon, Filippo, Thomas, Caitlyn A, Sannio, Filomena, Corsica, Giuseppina, Benvenuti, Manuela, Feller, Georges, Coulon, Rémi, Marcoccia, Francesca, Devente, Savannah Rowane, Bouajila, Ezeddine, Piveteau, Catherine, Leroux, Florence, Deprez-Poulain, Rebecca, Deprez, Benoît, Licznar-Fajardo, Patricia, Crowder, Michael W, Cendron, Laura, Pozzi, Cecilia, Mangani, Stefano, Docquier, Jean-Denis, Hernandez, Jean-François, Gavara, Laurent |
| المساهمون: | Legru, Alice, Verdirosa, Federica, Vo-Hoang, Yen, Tassone, Giusy, Vascon, Filippo, Thomas, Caitlyn A, Sannio, Filomena, Corsica, Giuseppina, Benvenuti, Manuela, Feller, George, Coulon, Rémi, Marcoccia, Francesca, Devente, Savannah Rowane, Bouajila, Ezeddine, Piveteau, Catherine, Leroux, Florence, Deprez-Poulain, Rebecca, Deprez, Benoît, Licznar-Fajardo, Patricia, Crowder, Michael W, Cendron, Laura, Pozzi, Cecilia, Mangani, Stefano, Docquier, Jean-Deni, Hernandez, Jean-Françoi, Gavara, Laurent |
| بيانات النشر: | AMER CHEMICAL SOC |
| سنة النشر: | 2022 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| الوصف: | Metallo-fi-lactamases (MBLs) contribute to the resistance of Gram-negative bacteria to carbapenems, last-resort antibiotics at hospital, and MBL inhibitors are urgently needed to preserve these important antibacterial drugs. Here, we describe a series of 1,2,4-triazole-3-thione-based inhibitors displaying an a- amino acid substituent, which amine was mono-or disubstituted by (hetero)aryl groups. Compounds disubstituted by certain nitrogen-containing heterocycles showed submicromolar activities against VIM-type enzymes and strong NDM-1 inhibition (Ki= 10-30 nM). Equilibrium dialysis, native mass spectrometry, isothermal calorimetry (ITC), and X-ray crystallography showed that the compounds inhibited both VIM-2 and NDM-1 at least partially by stripping the catalytic zinc ions. These inhibitors also displayed a very potent synergistic activity with meropenem (16-to 1000-fold minimum inhibitory concentration (MIC) reduction) against VIM-type-and NDM-1-producing ultraresistant clinical isolates, including Enterobacterales and Pseudomonas aeruginosa. Furthermore, selected compounds exhibited no or moderate toxicity toward HeLa cells, favorable absorption, distribution, metabolism, excretion (ADME) properties, and no or modest inhibition of several mammalian metalloenzymes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/36450011; info:eu-repo/semantics/altIdentifier/wos/WOS:000892648400001; volume:65; issue:24; firstpage:16392; lastpage:16419; numberofpages:28; journal:JOURNAL OF MEDICINAL CHEMISTRY; https://hdl.handle.net/11577/3465209Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85143550301 |
| DOI: | 10.1021/acs.jmedchem.2c01257 |
| الإتاحة: | https://doi.org/10.1021/acs.jmedchem.2c01257Test https://hdl.handle.net/11577/3465209Test |
| رقم الانضمام: | edsbas.5A4492F1 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1021/acs.jmedchem.2c01257 |
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