دورية أكاديمية
Regulatory cis- and trans-elements of mitochondrial D-loop-driven reporter genes in budding tunicates
العنوان: | Regulatory cis- and trans-elements of mitochondrial D-loop-driven reporter genes in budding tunicates |
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المؤلفون: | Kawamura, Kaz, Saitoh, Yuhya, Sunanaga, Takeshi, BALLARIN, LORIANO |
المساهمون: | Kawamura, Kaz, Saitoh, Yuhya, Ballarin, Loriano, Sunanaga, Takeshi |
بيانات النشر: | Elsevier B.V. |
سنة النشر: | 2017 |
المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
مصطلحات موضوعية: | 16S ribosomal RNA, Ascidian, Green fluorescent protein, Histone methylation, Non-coding region, TFAM, Molecular Medicine, Molecular Biology, Cell Biology |
الوصف: | To unveil the underlying mechanism of mitochondrial gene regulation associated with ageing and budding in the tunicate Polyandrocarpa misakiensis, mitochondrial non-coding-region (NCR)-containing reporter genes were constructed. PmNCR2.3K/GFP was expressed spatiotemporally in a pattern quite similar to mitochondrial 16SrRNA. The reporter gene expression was sensitive to high dose of rifampicin similar to mitochondrial genes, suggesting that the transcription indeed occurs in mitochondria. However, the gene expression also occurred in vivo in the cell nucleus and in vitro in the nuclear extracts. Mitochondrial transcription factor A (PmTFAM) enhanced reporter gene expression, depending on the NCR length. A budding-specific polypeptide TC14-3 is an epigenetic histone methylation inducer. It heavily enhanced reporter gene expression that was interfered by histone methylation inhibitors and PmTFAM RNAi. Our results indicate for the first time that the nuclear histone methylation is involved in mitochondrial gene activity via TFAM gene regulation. |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | STAMPA |
اللغة: | English |
العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/2852633; info:eu-repo/semantics/altIdentifier/wos/WOS:000405064700008; volume:35; firstpage:59; lastpage:69; numberofpages:11; journal:MITOCHONDRION; http://hdl.handle.net/11577/3237715Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85019831926 |
DOI: | 10.1016/j.mito.2017.05.006 |
الإتاحة: | https://doi.org/10.1016/j.mito.2017.05.006Test http://hdl.handle.net/11577/3237715Test |
رقم الانضمام: | edsbas.F8928996 |
قاعدة البيانات: | BASE |
DOI: | 10.1016/j.mito.2017.05.006 |
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