دورية أكاديمية
Neuropeptide S-initiated sequential cascade mediated by OX1, NK1, mGlu5 and CB1 receptors: A pivotal role in stress-induced analgesia
| العنوان: | Neuropeptide S-initiated sequential cascade mediated by OX1, NK1, mGlu5 and CB1 receptors: A pivotal role in stress-induced analgesia |
|---|---|
| المؤلفون: | Lee M. T., Chiu Y. -T., Chiu Y. -C., Hor C. C., Lee H. -J., Guerrini R., Calo' G., Chiou L. -C. |
| المساهمون: | Lee, M. T., Chiu, Y. -T., Chiu, Y. -C., Hor, C. C., Lee, H. -J., Guerrini, R., Calo', G., Chiou, L. -C. |
| بيانات النشر: | BioMed Central Ltd. |
| سنة النشر: | 2020 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: | Endocannabinoid, Metabotropic glutamate receptor, Neuropeptide S, Orexin, Periaqueductal gray, Substance P, Animal, Male, Mice, Neuropeptide, Orexin Receptor, Receptor, Cannabinoid, CB1, Metabotropic Glutamate 5, Stress, Psychological, Ventral Thalamic Nuclei, Analgesia |
| الوصف: | Background: Stress-induced analgesia (SIA) is an evolutionarily conserved phenomenon during stress. Neuropeptide S (NPS), orexins, substance P, glutamate and endocannabinoids are known to be involved in stress and/or SIA, however their causal links remain unclear. Here, we reveal an unprecedented sequential cascade involving these mediators in the lateral hypothalamus (LH) and ventrolateral periaqueductal gray (vlPAG) using a restraint stress-induced SIA model. Methods: Male C57BL/6 mice of 8-12 week-old were subjected to intra-cerebroventricular (i.c.v.) and/or intra-vlPAG (i.pag.) microinjection of NPS, orexin-A or substance P alone or in combination with selective antagonists of NPS receptors (NPSRs), OX1 receptors (OX1Rs), NK1 receptors (NK1Rs), mGlu5 receptors (mGlu5Rs) and CB1 receptors (CB1Rs), respectively. Antinociceptive effects of these mediators were evaluated via the hot-plate test. SIA in mice was induced by a 30-min restraint stress. NPS levels in the LH and substance P levels in vlPAG homogenates were compared in restrained and unrestrained mice. Results: NPS (i.c.v., but not i.pag.) induced antinociception. This effect was prevented by i.c.v. blockade of NPSRs. Substance P (i.pag.) and orexin-A (i.pag.) also induced antinociception. Substance P (i.pag.)-induced antinociception was prevented by i.pag. Blockade of NK1Rs, mGlu5Rs or CB1Rs. Orexin-A (i.pag.)-induced antinociception has been shown previously to be prevented by i.pag. blockade of OX1Rs or CB1Rs, and here was prevented by NK1R or mGlu5R antagonist (i.pag.). NPS (i.c.v.)-induced antinociception was prevented by i.pag. blockade of OX1Rs, NK1Rs, mGlu5Rs or CB1Rs. SIA has been previously shown to be prevented by i.pag. blockade of OX1Rs or CB1Rs. Here, we found that SIA was also prevented by i.c.v. blockade of NPSRs or i.pag. blockade of NK1Rs or mGlu5Rs. Restrained mice had higher levels of NPS in the LH and substance P in the vlPAG than unrestrained mice. Conclusions: These results suggest that, during stress, NPS is released and ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | ELETTRONICO |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31915019; info:eu-repo/semantics/altIdentifier/wos/WOS:000512121200001; volume:27; issue:1; firstpage:1; lastpage:15; numberofpages:15; journal:JOURNAL OF BIOMEDICAL SCIENCE; http://hdl.handle.net/11577/3386670Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85077704312 |
| DOI: | 10.1186/s12929-019-0590-1 |
| الإتاحة: | https://doi.org/10.1186/s12929-019-0590-1Test http://hdl.handle.net/11577/3386670Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.DB503579 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s12929-019-0590-1 |
|---|