دورية أكاديمية
PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis
| العنوان: | PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis |
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| المؤلفون: | Mana Giulia, Clapero Fabiana, Panieri Emiliano, Panero Valentina, Böttcher Ralph T, Tseng Hui Yuan, Saltarin Federico, Astanina Elena, Wolanska Katarzyna I, Morgan Mark R, Humphries Martin J, Serini Guido, Valdembri Donatella, SANTORO, MASSIMO |
| المساهمون: | Mana, Giulia, Clapero, Fabiana, Panieri, Emiliano, Panero, Valentina, Böttcher Ralph, T, Tseng Hui, Yuan, Saltarin, Federico, Astanina, Elena, Wolanska Katarzyna, I, Morgan Mark, R, Humphries Martin, J, Santoro, Massimo, Serini, Guido, Valdembri, Donatella |
| سنة النشر: | 2016 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: | Integrin, Angiogenesi, Endothelial Cell, Endocytosi, Trans-Golgi network, Fibronectin, Embryonic development, Zebrafish |
| الوصف: | Basolateral polymerization of cellular fibronectin (FN) into a meshwork drives endothelial cell (EC) polarity and vascular remodelling. However, mechanisms coordinating α5β1 integrin-mediated extracellular FN endocytosis and exocytosis of newly synthesized FN remain elusive. Here we show that, on Rab21-elicited internalization, FN-bound/active α5β1 is recycled to the EC surface. We identify a pathway, comprising the regulators of post-Golgi carrier formation PI4KB and AP-1A, the small GTPase Rab11B, the surface tyrosine phosphatase receptor PTPRF and its adaptor PPFIA1, which we propose acts as a funnel combining FN secretion and recycling of active α5β1 integrin from the trans-Golgi network (TGN) to the EC surface, thus allowing FN fibrillogenesis. In this framework, PPFIA1 interacts with active α5β1 integrin and localizes close to EC adhesions where post-Golgi carriers are targeted. We show that PPFIA1 is required for FN polymerization-dependent vascular morphogenesis, both in vitro and in the developing zebrafish embryo. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/27876801; info:eu-repo/semantics/altIdentifier/wos/WOS:000388323000001; volume:7; firstpage:13546; lastpage:13565; numberofpages:20; journal:NATURE COMMUNICATIONS; http://hdl.handle.net/11577/3242244Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84997108022; http://www.nature.com/articles/ncomms13546Test |
| DOI: | 10.1038/ncomms13546 |
| الإتاحة: | https://doi.org/10.1038/ncomms13546Test http://hdl.handle.net/11577/3242244Test http://www.nature.com/articles/ncomms13546Test |
| رقم الانضمام: | edsbas.71D7A38C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/ncomms13546 |
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