دورية أكاديمية
Lactobacillus crispatus M247-Derived H2O2 Acts as a Signal Transducing Molecule Activating Peroxisome Proliferator Activated Receptor-γ in the Intestinal Mucosa
| العنوان: | Lactobacillus crispatus M247-Derived H2O2 Acts as a Signal Transducing Molecule Activating Peroxisome Proliferator Activated Receptor-γ in the Intestinal Mucosa |
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| المؤلفون: | VOLTAN, SANDRA, MARTINES, DIEGO, BRUN, PAOLA, PORZIONATO, ANDREA, MACCHI, VERONICA, D'INCA', RENATA, SCARPA, MELANIA, PALU', GIORGIO, STURNIOLO, GIACOMO, MORELLI, LORENZO, CASTAGLIUOLO, IGNAZIO, Elli, Marina, Longo, Stefano |
| المساهمون: | Voltan, Sandra, Martines, Diego, Elli, Marina, Brun, Paola, Longo, Stefano, Porzionato, Andrea, Macchi, Veronica, D'Inca', Renata, Scarpa, Melania, Palu', Giorgio, Sturniolo, Giacomo, Morelli, Lorenzo, Castagliuolo, Ignazio |
| سنة النشر: | 2008 |
| المجموعة: | Padua Research Archive (IRIS - Università degli Studi di Padova) |
| مصطلحات موضوعية: | Animal, Cell Line, Coliti, Colon, Cytokine, Epithelial Cell, Free Radical, Hydrogen Peroxide, Immunologic Factor, In Vitro Technique, Intestinal Mucosa, Lactobacillu, Male, Mice, Inbred C57BL, Mutant Strain, PPAR gamma, Probiotic, Signal Transduction, Transcription, Genetic, Transfection, Gastroenterology |
| الوصف: | BACKGROUND & AIMS: Accumulating evidence indicates that the peroxisome proliferator activated receptor (PPAR)-gamma is a major player in maintaining intestinal mucosa homeostasis, but whether PPAR-gamma is directly involved in probiotic-mediated effects and the molecular events involved in its activation are not known. METHODS: We investigated the role of PPAR-gamma in the immunomodulatory effects of Lactobacillus crispatus M247 on intestinal epithelial cells (IEC) and the role of probiotic-derived H(2)O(2) on PPAR-gamma activity. RESULTS: L crispatus M247 supplementation in mice significantly increased PPAR-gamma levels and transcriptional activity in the colonic mucosa. L crispatus M247 induced PPAR-gamma nuclear translocation and enhanced transcriptional activity in epithelial (CMT-93) cells, as demonstrated by the increased luciferase activity of a PPAR-gamma-responsive element, PPAR-gamma-responsive gene up-regulation, and reduced activity of an nuclear factor-kappaB-responsive element. Pharmacologic PPAR-gamma inhibition or silencing by small interfering RNA cancelled the L crispatus M247-mediated effects in CMT-93 cells. Because Lactobacillus strains producing little H(2)O(2) failed to activate PPAR-gamma, we investigated the role of L crispatus M247-derived H(2)O(2) in PPAR-gamma activation. L crispatus M247 induced a transient rise in intracellular H(2)O(2) and PPAR-gamma transcriptional activity was cancelled by antioxidant or H(2)O(2) scavenger. Toll-like receptor (TLR)-2 was not required for PPAR-gamma up-regulation mediated by L crispatus M247 in mice, although the protective effects of L crispatus M247 on dextran sodium sulfate-induced colitis were less pronounced in TLR-2(-/-) mice. CONCLUSIONS: L crispatus M247 uses H(2)O(2) as a signal transducing molecule to induce PPAR-gamma activation in IEC, directly modulating epithelial cell responsiveness to inflammatory stimuli. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | STAMPA |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/18722375; info:eu-repo/semantics/altIdentifier/wos/WOS:000259982800030; volume:135; issue:4; firstpage:1216; lastpage:1227; numberofpages:12; journal:GASTROENTEROLOGY; http://hdl.handle.net/11577/3214609Test; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-53049106239 |
| DOI: | 10.1053/j.gastro.2008.07.007 |
| الإتاحة: | https://doi.org/10.1053/j.gastro.2008.07.007Test http://hdl.handle.net/11577/3214609Test |
| رقم الانضمام: | edsbas.81886245 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1053/j.gastro.2008.07.007 |
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