التفاصيل البيبلوغرافية
| العنوان: |
Protein-protein interaction site mapping using NMR-detected mutational scanning. |
| المؤلفون: |
Baminger, B, Ludwiczek, M, Kontaxis, G, Knapp, S, Konrat, R |
| سنة النشر: |
2016 |
| المجموعة: |
Oxford University Research Archive (ORA) |
| مصطلحات موضوعية: |
genetics, Reproducibility of Results, Protein Interaction Mapping, Models, Molecular, TCF Transcription Factors, beta Catenin, Transcription Factor 7-Like 2 Protein, Ligands, methods, Binding, Competitive, metabolism, Nuclear Magnetic Resonance, Biomolecular, Mutation |
| الوصف: |
We demonstrate a novel NMR method for the mapping of protein-protein interaction sites. In our approach protein-protein binding sites are mapped by competition binding experiments using indirect NMR reporter technology and Ala positional scanning. The methodology provides high sensitivity, ease of implementation and high-throughput capabilities. The feasibility of the technique is demonstrated with an application to the beta-Catenin/Tcf4 complex. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
https://ora.ox.ac.uk/objects/uuid:cbeced7b-bf5e-4782-be0c-00d4bc852333Test |
| الإتاحة: |
https://ora.ox.ac.uk/objects/uuid:cbeced7b-bf5e-4782-be0c-00d4bc852333Test |
| حقوق: |
info:eu-repo/semantics/embargoedAccess |
| رقم الانضمام: |
edsbas.5CF1963C |
| قاعدة البيانات: |
BASE |
