دورية أكاديمية
Non-classical ProIL-1beta activation during mammary gland infection is pathogen-dependent but Caspase-1 independent
| العنوان: | Non-classical ProIL-1beta activation during mammary gland infection is pathogen-dependent but Caspase-1 independent |
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| المؤلفون: | Breyne, Koen, Cool, Steven, Demon, Dieter, Demeyre, Kristel, Vandenberghe, Tom, Vandenabeele, Peter, Carlsen, Harald, Van Den Broeck, Wim, Sanders, Niek, Meyer, Evelyne |
| سنة النشر: | 2015 |
| المجموعة: | Open archive Norwegian University of Life Sciences (NMBU) |
| الوصف: | Infection of the mammary gland with live bacteria elicits a pathogen-specific host inflammatory response. To study these host-pathogen interactions wild type mice, NF-kappaB reporter mice as well as caspase-1 and IL-1beta knockout mice were intramammarily challenged with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The murine mastitis model allowed to compare the kinetics of the induced cytokine protein profiles and their underlying pathways. In vivo and ex vivo imaging showed that E. coli rapidly induced NF-kappaB inflammatory signaling concomitant with high mammary levels of TNF-alpha, IL-1 alpha and MCP-1 as determined by multiplex analysis. In contrast, an equal number of S. aureus bacteria induced a low NF-kappaB activity concomitant with high mammary levels of the classical IL-1beta fragment. These quantitative and qualitative differences in local inflammatory mediators resulted in an earlier neutrophil influx and in a more extensive alveolar damage post-infection with E. coli compared to S. aureus. Western blot analysis revealed that the inactive proIL-1beta precursor was processed into pathogen-specific IL-1beta fragmentation patterns as confirmed with IL-1beta knockout animals. Additionally, caspase-1 knockout animals allowed to investigate whether IL-1beta maturation depended on the conventional inflammasome pathway. The lack of caspase-1 did not prevent extensive proIL-1beta fragmentation by either of S. aureus or E. coli. These non-classical IL-1beta patterns were likely caused by different proteases and suggest a sentinel function of IL-1beta during mammary gland infection. Thus, a key signaling nodule can be defined in the differential host innate immune defense upon E. coli versus S. aureus mammary gland infection, which is independent of caspase-1. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | PLoS ONE 2014, 9(8); urn:issn:1932-6203; http://hdl.handle.net/11250/282569Test; https://doi.org/10.1371/journal.pone.0105680Test; cristin:1204835 |
| DOI: | 10.1371/journal.pone.0105680 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0105680Test http://hdl.handle.net/11250/282569Test |
| حقوق: | Navngivelse 3.0 Norge ; http://creativecommons.org/licenses/by/3.0/noTest/ |
| رقم الانضمام: | edsbas.93D35397 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0105680 |