رسالة جامعية
Loss or down-regulation of HLA class I expression at the allelic level in freshly isolated leukemic blasts ; 白血病細胞におけるHLAクラスIの発現欠失・低下についての検討
| العنوان: | Loss or down-regulation of HLA class I expression at the allelic level in freshly isolated leukemic blasts ; 白血病細胞におけるHLAクラスIの発現欠失・低下についての検討 |
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| المؤلفون: | Masuda, Kozo |
| سنة النشر: | 2016 |
| المجموعة: | Okayama University Scientific Achievement Repository / 岡山大学学術成果リポジトリ |
| مصطلحات موضوعية: | REVERSE-TRANSCRIPTASE, HISTOCOMPATIBILITY LEUKOCYTE ANTIGEN, TELOMERASE, CHRONIC MYELOID-LEUKEMIA, CYTOTOXIC T-LYMPHOCYTES, TUMOR-CELL LINES, HAPLOTYPE LOSS, LARYNGEAL CARCINOMAS, GENETIC ALTERATIONS, SURFACE EXPRESSION, CERVICAL-CANCER |
| الوصف: | Loss or down-regulation of human leukocyte antigen (HLA) class I expression has been demonstrated in a variety of solid tumors. To date, such altered HLA expression has not been studied extensively in freshly isolated leukemic blasts. If it occurs, leukemic cells could escape T-cell surveillance as a consequence. Genotypes of nine leukemic cell lines were determined using a polymerase chain reaction for HLA classes I and II. Cells were also examined for HLA beta2-microglobulin, and allele-specific HLA protein expression using flow cytometry. Next, 44 samples of freshly isolated leukemic blasts from 43 patients with malignant hematological diseases were examined for allele-specific HLA expression using flow cytometry. Microsatellite analysis was performed to determine heterozygosity in the HLA region on chromosome 6. Genotype analysis for HLA class I together with microsatellite analysis demonstrated loss of HLA haplotype in HL-60 cells. No loss of HLA haplotype was observed in 44 samples of freshly isolated leukemic blasts. As reported previously, flow cytometric analysis rarely demonstrated loss or down-regulation of HLA expression at initial diagnosis (3/39; 7.7%); however, this was evident in two of five cases in relapse (40.0%), which contrasts with previous reports. In one patient with acute leukemia, HLA-A2 cell surface expression was present at initial diagnosis, lost at relapse, and completely restored after 48 h of culture in the presence of interferon-gamma. These results suggest loss of allele-specific HLA expression may be involved in the pathogenesis of relapse in patients with leukemia. The findings should be valuable in designing new strategies for clinical immunotherapy. |
| نوع الوثيقة: | thesis |
| اللغة: | Japanese |
| العلاقة: | https://ousar.lib.okayama-u.ac.jp/files/public/1/11720/20160527190041879432/K003313.pdfTest; https://ousar.lib.okayama-u.ac.jp/11720Test |
| الإتاحة: | https://ousar.lib.okayama-u.ac.jp/files/public/1/11720/20160527190041879432/K003313.pdfTest https://ousar.lib.okayama-u.ac.jp/11720Test |
| رقم الانضمام: | edsbas.AB193428 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |