دورية أكاديمية
Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus
| العنوان: | Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus |
|---|---|
| المؤلفون: | Driche, El Hadj, Sabaou, Nasserdine, Bijani, Christian, Zitouni, Abdelghani, Pont, Frédéric, Mathieu, Florence, Badji, Boubekeur |
| المساهمون: | Centre National de la Recherche Scientifique - CNRS (FRANCE), Ecole normale supérieure de Kouba (ALGERIA), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National de la Santé et de la Recherche Médicale - INSERM (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Université Hassiba Benbouali de Chlef (ALGERIA), Laboratoire de Chimie de Coordination - LCC (Toulouse, France) |
| بيانات النشر: | Springer |
| سنة النشر: | 2017 |
| المجموعة: | OATAO (Open Archive Toulouse Archive Ouverte - Université de Toulouse) |
| مصطلحات موضوعية: | Génie chimique, Génie des procédés, Antibiofilm, Furanone derivative, Multidrug-resistant, Staphylococcus aureus, Saharan soil, Streptomyces |
| الوصف: | A novel actinobacterium strain, named AT37, showed a strong activity against some multidrug-resistant Staphylococcus aureus, including methicillin-resistant S. aureus MRSA ATCC 43300, other clinical isolates of MRSA and vancomycin resistant S. aureus VRSA S1. The strain AT37 was isolated from a Saharan soil by a dilution agar plating method using chitin-vitamin agar medium supplemented with rifampicin. The morphological and chemical studies indicated that this strain belonged to the genus Streptomyces. Its 16S rRNA gene sequence was determined and a database search indicated that it was closely associated with the type strain of Streptomyces novaecaesareae NBRC 13368T with 99.6% of similarity. However, the comparison of the morphological and the physiological characteristics of the strain with those of the nearest species showed significant differences. The strain AT37 secreted the antibiotic optimally during mid-stationary phase of growth. One active compound (AT37-1) was extracted from the culture broth with dichloromethane, separated on silica gel plates and purified by HPLC. Based on spectroscopic analysis of UV-Visible, infrared, and 1H and 13C NMR spectra and spectrometric analysis, the chemical structure of the compound AT37-1 was identified as 5-[(5E,7E,11E)-2,10-dihydroxy-9,11-dimethyl-5,7,11-tridecatrien-1-yl]-2-hydroxy-2-(1-hydroxyethyl)-4-methyl-3(2H)-furanone. Minimum inhibitory concentrations and minimum biofilm inhibitory concentration (MBIC50) of this compound showed significant activity against multidrug-resistant S. aureus with 15-30 and 10-15 μg/mL, respectively. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://oatao.univ-toulouse.fr/20389/1/Driche_20389.pdfTest; HAL : hal-01948255; Driche, El Hadj and Sabaou, Nasserdine and Bijani, Christian and Zitouni, Abdelghani and Pont, Frédéric and Mathieu, Florence and Badji, Boubekeur. Streptomyces sp. AT37 isolated from a Saharan soil produces a furanone derivative active against multidrug-resistant Staphylococcus aureus. (2017) World Journal of Microbiology and Biotechnology, 33 (6). 1-13. ISSN 0959-3993 |
| DOI: | 10.1007/s11274-017-2265-y |
| الإتاحة: | https://doi.org/10.1007/s11274-017-2265-yTest https://oatao.univ-toulouse.fr/20389Test/ https://oatao.univ-toulouse.fr/20389/1/Driche_20389.pdfTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.62B7AFAF |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s11274-017-2265-y |
|---|