كتاب
A Hyperstable Miniprotein: Additive Effects of D- and L-Ala Substitutions
| العنوان: | A Hyperstable Miniprotein: Additive Effects of D- and L-Ala Substitutions |
|---|---|
| المؤلفون: | D. V. Williams, Bipasha Barua, Niels H. Andersen |
| المصدر: | Nature Precedings |
| سنة النشر: | 2008 |
| المجموعة: | Nature Precedings |
| مصطلحات موضوعية: | Chemistry |
| الوصف: | The effects of alanine substitutions in each helical segment of the structure, and Gly to D-Ala mutations at sites where glycines have positive phi angles in the Trp-cage miniprotein are reported. The effects of the stabilizing mutation were additive, yielding a 20-residue construct (Tm = 83^o^C). Gly to L-Ala substitutions were uniformly destabilizing ([DELTA][DELTA]G~F~ > 11 kJ/mol): the preference for a D-Ala can be as large as 16 kJ/mol. Glycine to D-Ala mutations are validated as a strategy for the design of hyperstable miniprotein scaffolds suitable for stereospecific pharmacophore display. |
| نوع الوثيقة: | manuscript |
| اللغة: | unknown |
| العلاقة: | http://precedings.nature.com/documents/2074/version/1Test; oai:nature.com:10101/npre.2008.2074.1; http://hdl.handle.net/10101/npre.2008.2074.1Test |
| الإتاحة: | http://precedings.nature.com/documents/2074/version/1Test http://hdl.handle.net/10101/npre.2008.2074.1Test |
| حقوق: | Creative Commons Attribution 3.0 License |
| رقم الانضمام: | edsbas.51CB1DA4 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |