تقرير
養殖蝦抗病能力之調控II - 抗病基因產物之篩選
| العنوان: | 養殖蝦抗病能力之調控II - 抗病基因產物之篩選 |
|---|---|
| المؤلفون: | 宋延齡 |
| بيانات النشر: | 臺北市：國立臺灣大學動物學系暨研究所 |
| سنة النشر: | 2001 |
| المجموعة: | National Taiwan University Institutional Repository (NTUR) |
| مصطلحات موضوعية: | 草蝦, 乳鐵蛋白, 乳鐵蛋白N:11 mer, 殺菌活性, Tiger shrimp, antimicrobial activities, bovine lactoferrin (BLF), N: 11 mer |
| جغرافية الموضوع: | 計畫年度：89 第二期, 起迄日期：2000-08-01/2001-07-31 |
| الوصف: | 由於蝦病繁生，自1987 年以來養殖草 蝦的產量每下愈況，改善此困境的方法之 一為加強宿主本身的抗病能力。乳鐵蛋白 (lactoferrin) 是一種可與鐵結合的醣蛋 白，最早由牛奶中純化出來，具有殺菌活 性以及調節免疫反應等多項生理功能；N: 11 mer 為乳鐵蛋白N 端的11 個氨基酸片 段，亦具殺菌及免疫刺激的活性；生長激 素(Growth hormone, GH) 由脊椎動物的腦 下垂體前葉所分泌，可刺激動物生長；哺 乳動物之Interlukin-1β (IL-1β) 在不同的 細胞中具有不同的功能，主要做為調控免 疫反應的物質。本實驗選取乳鐵蛋白、乳 鐵蛋白N: 11 mer 、yellowfin progy ( Acanthopagrus latus) recombinant growth hormone (ypGH) 、a-Vibrio Ig 及carp ( Cyprinus carpio) recombinant interlukin-1b (cIL-1b) 等物質以離體實驗進行免疫調節 或抗病功能評估，以做為選取標的抗病基 因進行基因轉殖蝦實驗的基本資料。 實驗結果顯示乳鐵蛋白、乳鐵蛋白N: 11 mer、 ypGH 以及cIL-1β皆可促進蝦血 球細胞產生O2 -，O2 -為吞噬作用的最初產 物。在抑菌作用方面，不論乳鐵蛋白或是 乳鐵蛋白N: 11 mer 均可抑制蝦病原弧菌 的生長；至於對血球細胞膜的影響，乳鐵 蛋白與血球培養3 小時，乳鐵蛋白N: 11 mer 與血球培養1 小時後，血球存活率均較 對照組顯著的降低。 ; To investigate the peptide-antimicrobial activity relationship of shrimp, bovine lactoferrin (BLF), the basic amino acid-rich region of lactoferricin B, RRWQWRMKKLG (N: 11 mer), yellowfin progy ( Acanthopagrus latus) recombinant growth hormone (ypGH) and carp ( Cyprinus carpio) recombinant interlukin-1b (cIL-1b) were selected. Tiger shrimp ( Penaeus monodon) hemocytes were incubated with bLF, N: 11 mer, ypGH and cIL-1b, respectively, and quantified the production of superoxide anion (O2 -) by nitroblue tetrazolium (NBT) staining. All of the peptides could enhance the production of O2 - of shrimp hemocytes, which was the first product of phagocytosis. The antimicrobial activities of the peptides were also tested by determining the minimal inhibitory concentration (MIC) of Vibrio vulnificus, Vibrio alginolyticus and Vibrio harveyi, respectively, in the presence of the peptides. In contrast to ypGH and cIL-1b, bLF and N: 11 mer were successful to inhibit the growth of the pathogen of tiger shrimp. Finally, the membrane disruptions of tiger shrimp hemocytes that caused by bLF and N: 11 mer, respectively, were determined with trypan blue staining. The survival rate of bLF- and N: 11 mer-treated hemocytes was significant differ with untreated hemocytes after 3- or 1-hour (s) incubation, respectively. These results suggest that bLF and N: 11 mer ... |
| نوع الوثيقة: | report |
| وصف الملف: | application/pdf; 127060 bytes |
| اللغة: | Chinese |
| العلاقة: | 892313B002232; http://ntur.lib.ntu.edu.tw/handle/246246/16812Test; http://ntur.lib.ntu.edu.tw/bitstream/246246/16812/1/892313B002232.pdfTest |
| الإتاحة: | http://ntur.lib.ntu.edu.tw/handle/246246/16812Test http://ntur.lib.ntu.edu.tw/bitstream/246246/16812/1/892313B002232.pdfTest |
| حقوق: | 國立臺灣大學動物學系暨研究所 |
| رقم الانضمام: | edsbas.BA5CCE03 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |