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自組裝薄膜分離與電流式分子感測之研究 ; A Study of Self-Assembling Membrane Separation and Amperometric Molecular Sensing

التفاصيل البيبلوغرافية
العنوان: 自組裝薄膜分離與電流式分子感測之研究 ; A Study of Self-Assembling Membrane Separation and Amperometric Molecular Sensing
المؤلفون: 陳宏杰, Chen, Hung-Chieh
المساهمون: 何國川, 臺灣大學：化學工程學研究所
سنة النشر: 2004
المجموعة: National Taiwan University Institutional Repository (NTUR)
مصطلحات موضوعية: 電化學感測, 嗎啡, 半胱胺酸, SAM薄膜分離, 修飾電極, morphine, modified electrode, SAM membrane separation, cysteine, electrochemical detection
الوصف: 有鑑於一般的生化感測方法往往具有昂貴、體積大、保存不易及較長的分析感測時間等不利因素，本研究使用修飾薄膜及修飾電極分別利用薄膜分離與電化學感測方法，試圖發展出體積小、價格低且感測時間短的生化感測器。本研究主要成果將分別說明如下。 在薄膜分離實驗中，以流體透過法、SEM及AFM分析PCTE (polycarbonate track-etched)多孔性薄膜之性質，其孔洞有效半徑為21±1 nm，孔洞密度為7.5x108 pores/cm2。利用無電鍍法鍍金，使PCTE薄膜具有導電性，平均鍍金速率約為8~9 nm/hr，當施加電位於鍍金之PCTE薄膜，可以調控帶電離子之透過流率。此外，將鍍金薄膜浸泡於半胱胺 (cysteine, Cys)溶液中，可製備自組裝單層 (self-assembling monolayer, SAM)修飾薄膜，藉由溶液中pH值的調整，可改變SAM修飾薄膜之表面電荷分佈，藉此影響標的物在薄膜透過的流率，達到選擇性分離。本研究分別以甲基藍、甲基橙及嗎啡來驗證此SAM薄膜，由結果顯示分別在高pH值、低pH值及中性pH值溶液中有最大的透過流率。 利用電化學方法感測嗎啡的實驗中，由循環伏安 (cyclic voltammety, 簡稱CV)法得知於高濃度的碘離子溶液中才能明顯測出碘可促進嗎啡之氧化而提高電流響應。定電位感測嗎啡時，在0.1 M碘離子溶液中，選擇感測電位為0.65 V (vs. Ag/AgCl Sat’d KCl)，取樣時間為150秒下，得到感測之線性範圍為100μM~600μM，感測靈敏度為3.69μA/cm2-μM。嗎啡反應後之產物為白色粉末沈澱，將其沖洗、乾燥後經1H NMR及MS分析，證實此白色粉末為擬嗎啡(pseudomorphine)，此與文獻中利用電化學氧化嗎啡的產物是相同的。 此外，本研究亦利用鐵氰化鎳 (nickel hexacyanoferrate, 簡稱NiHCF)修飾電極氧化感測Cys，以CV法將NiHCF析鍍於FTO導電玻璃基材上，並於水溶液中添加Ni2+離子，可以有效抑製氧化態NiHCF之溶解反應，提升FTO/NiHCF工作電極之穩定性。以定電位方式感測Cys時，溶液中添加15 mM Ni2+離子及0.1 M KH2PO4，選擇感測電位為0.8 V (vs. Ag/AgCl Sat’d KCl)，取樣時間為50秒下，感測之線性範圍為150μM~1 mM，此感測線性範圍包含了Cys在人體之正常濃度範圍。 綜合上述之研究成果，SAM修飾薄膜提供一體積小及操作簡便之分離方法；利用修飾電極搭配電化學感測方式，亦具備了操作簡便、感測時間短、材料價格較低及保存容易等優點。相信本研究對於生化感測之多元發展將有所幫助。 ; Due to the common problems found in bio-analytical methods, in which high cost, large instrument, uneasy storage and time consuming are encountered. This research tries to develop an inexpensive, small and fast response analyzing method by a modified membrane and a modified electrode. Major results of this research are summarized in the following paragraphes. In polycarbonate track-etched (PCTE) porous membrane, its basic separation properties were measured by the fluid permeation, SEM and AFM. The results showed that the effective pore radius was 21±1 nm and the pore density was 7.5x108 pores/cm2. By using an electroless plating method, the surface of the PCTE membrane was covered with a conductive golden thin film. The average electroless plating rate was around 8~9 nm/hr. Based on this conductive golden-coated PCTE membrane, the permeated flux of the ions could be adjusted by an ...
نوع الوثيقة: thesis
وصف الملف: 2185020 bytes; application/pdf
اللغة: Chinese
English
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Stewart, and E. N. Lightfoot, “Transport Phenomena”, John Wiley & Sons, New York, pp. 8, 42-48 (1960).; zh-TW; http://ntur.lib.ntu.edu.tw/handle/246246/52238Test; http://ntur.lib.ntu.edu.tw/bitstream/246246/52238/1/ntu-93-R91524019-1.pdfTest
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http://ntur.lib.ntu.edu.tw/bitstream/246246/52238/1/ntu-93-R91524019-1.pdfTest
رقم الانضمام: edsbas.1FCAF1DE
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