التفاصيل البيبلوغرافية
| العنوان: |
評估基質輔助雷射脫附離子化－飛行時間質譜儀分型近平滑念珠菌群之可行性 ; Evaluation of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry for differentiation of Candida parapsilosis complex |
| المؤلفون: |
曾耀嬬, Yao-Ru Tseng |
| المساهمون: |
生命科學院碩士在職專班, 林正宏, Jeng-Horng Lin |
| سنة النشر: |
2015 |
| المجموعة: |
National Chung Hsing University Institutional Repository - NCHUIR / 國立中興大學 |
| مصطلحات موضوعية: |
近平滑念珠菌群, 基質輔助雷射脫附離子化－飛行時間質譜儀, 外顯子間填裝內含子之聚合酶連鎖反應, Candida parapsilosis complex, Matrix-assisted laser desorption / ionization time-of-flight mass spectrometry, Exon-primed intron-crossing polymerase chain reaction |
| الوصف: |
念珠菌菌血症病例在世界各醫院日益增加，尤其是免疫功能有缺陷或是重症的病人，需要接受免疫抑制劑或化學治療，長期的血液透析，以及全靜脈營養注射或是中央靜脈導管置放等侵入性醫療裝置，近平滑念珠菌群(Candida parapsilosis complex)藉由移生而造成病人的感染，甚至爆發群聚事件，因之念珠菌的鑑定與研究益形重要。Lehmann等人(1992)年利用randomly amplified polymorphic DNA 分析，將C. parapsilosis complex分為groupⅠ、II、Ⅲ。Tavanti等人(2005)年發現groupⅠ與group II及Ⅲ的Internal transcribed spacer(ITS1)基因序列相似度小於90%，認定他們為亞種，所以將group II 稱為Candida orthopsilosis，， groupⅢ則稱為Candida metapsilosis，此兩者的侵入性及毒性也有所不同。有鑑於鑑定方法推陳出新，本研究自2009年6月起至2014年8月止，收集來自中國醫藥大學附設醫院血液檢體分離出的C. parapsilosis complex，共101株。所有菌株皆以API ID32C商業套組鑑定結果為C. parapsilosis；樣品經蟻酸萃取後以基質輔助雷射脫附離子化－飛行時間(MALDI-TOF)質譜儀鑑定並分型，其結果為C. parapsilosis 75株(75/101)佔74%，C. orthopsilosis 11株(11/101)佔11 %，C. metapsilosis 15株(11/101)佔15 %。最後再以外顯子間填裝內含子之聚合酶連鎖反應(EPIC -PCR)驗證MALDI-TOF的結果，發現MALDI-TOF的結果與EPIC- PCR的結果100%符合。綜合前述本研究利用MALDI-TOF進行C. parapsilosis complex分型，進而證實MALDI-TOF能快速且準確區分C. parapsilosis complex。 ; Candida bacteremia cases increasing in various hospitals of the world, especially in immunocompromised, prior immunosuppressive therapy, chemotherapy, hemodialysis, total parenteral nutrition, central venous catheter injection , and other invasive medical devices, Candida parapsilosis complex caused by colonization and infection of patients, even the outbreak of nosocomial infection. In 1992, Lehmann et al. suggested that C. parapsilosis isolates can be divided into groupⅠ,Ⅱ, and Ⅲ on the basis of randomly amplified polymorphic DNA (RAPD) results. However, Tavanti et al. (2005) found C. parapsilosis groupⅠcompared with groupⅡand Ⅲ of DNA sequences similarities were less than 90% in the ITS1 sequence, and formally designate C. parapsilosis groups II and III as the new species C. orthopsilosis and C. metapsilosis on the grounds. This study included 101 C. parapsilosis complex strains identified among blood sample isolates collected from June 2009 to August 2014 in China Medical University Hospital. Identification of the C . parapsilosis was tested using API ID 32C .Formic acid extraction sample was identified by Matrix-assisted laser desorption / ... |
| نوع الوثيقة: |
thesis |
| اللغة: |
Chinese |
| العلاقة: |
U0005-1508201511500600; http://hdl.handle.net/11455/92623Test |
| الإتاحة: |
http://hdl.handle.net/11455/92623Test |
| حقوق: |
restricted |
| رقم الانضمام: |
edsbas.C0CB02D5 |
| قاعدة البيانات: |
BASE |
